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Thermal denaturation assays and size exclusion chromatography with multi-angle light scattering analyses show that Î RBD deletion does not influence the overall shape and multimerization of purified His-TRIM25Î RBD. Figure S2. Generation and characterization of TRIM25 KO HeLa cell line. Figure S3. T7-TRIM25 CLIP-seq and T7-TRIM25 RNA immunoprecipitation results. Figure S4. T7-TRIM25 CLIP-seq experiments are reproducible, based on three independent experiments. Figure S5. No correlation of TRIM25 CLIP-seq cluster intensities with transcript abundance in HeLa cells. Figure S6. Gene Ontology-term annotation of TRIM25-bound transcripts and the ab initio-derived TRIM25-binding binding sequences cluster in three motif clusters. Figure S7. TRIM25 does not influence RNA stability of selected targets. Figure S8. TRIM25 undergoes ubiquitination. (PDF 9873 kb)

COVID-19の世界的流行の中で，重篤な肺疾患に対し適用されるECMO治療に，一般社会からも注目が集まっている．本稿では，臨床工学技士が臨床の現場で携わるであろうECMO治療の概要やCOVID-19禍でのECMO治療適用の現状について紹介する．

В статье рассмотрены изменения в работе авиатранспорта в условиях распространения COVID-19. Проведена оценка влияния пандемии на развитие грузовых авиаперевозок. Изучены организации авиаперевозок на основе использования цифровой платформы ONE Source

A Bayesian analysis concludes beyond a reasonable doubt that SARS-CoV-2 is not a natural zoonosis but instead is laboratory derived.

The term digital literacy is rarely used in Japan. Instead, the terms computer literacy and ICT literacy are often used. In Europe and North America, the term digital literacy is commonly used as a skill for using information devices and the Internet. In the United States, digital literacy support in libraries is part of the national digital inclusion policy. Critical digital literacy has been strongly advocated in the wake of the "fake news" phenomenon. This theoretical trend has been influenced by critical literacy theory and media literacy theory. Digital and media literacy theory is a theory that integrates digital literacy and media literacy. This idea was advanced in theoretical research and practice by Renee Hobbs. In Japan, there is a lack of understanding of guaranteeing digital literacy as a right of citizens. In addition, digital and media literacy education and practice is very important in the era of COVID-19 and needs to be adapted to Japan.

Diese Notiz beschreibt die Veränderungen des Schweizer Verkehrsverhaltens durch die Einschränkungen zur Eindämmung der Sars-CoV-2 Pandemie. Die Auswertungen der Google oder Intervista Daten haben inhaltliche Grenzen, die wir durch weitere Befragungsinhalte nicht haben. Wir beschreiben unsere GPS tracking Daten in Kürze und konzentrieren uns auf die Darstellung ausgesuchter Veränderungen, insbesondere auf die nach Einkommensgruppen und Lage der Wohnungen. Diese Notiz wird durch die wöchentlichen Berichte auf https://ivtmobis.ethz.ch/mobis/covid19/reports ergänzt. This note describes the changes in travel behaviour due to the restrictions imposed to slow the spread of the Sars-CoV-2 epidemic in Switzerland. The existing analyses of the Google or the national Intervisa data have limits due to missing socio-demographic details about their users. We briefly describe our GPS tracking panel and its sample. The analyses focus on the changes for different income groups and for the differences in accessibility. It will be supplemented by the weekly https://ivtmobis.ethz.ch/mobis/covid19/reports. Arbeitsberichte Verkehrs- und Raumplanung, 1503

We study the interplay of non-pharmaceutical containment measures, human behavior, and the spread of COVID-19 in Switzerland. First, we collect sub-national data and construct indices that capture the stringency of containment measures at the cantonal level. Second, we use a vector autoregressive (VAR) model to analyze feedback effects between our variables of interest via structural impulse responses. Our results suggest that increases in the stringency of containment measures lead to a significant reduction of weekly infections as well as debit card transactions, which serve as a proxy for behavioral changes in the population. Furthermore, analyzing different policy measures individually shows that business closures, recommendations to work from home, and restrictions on gatherings have been particularly effective in containing the spread of COVID-19 in Switzerland. Finally, our findings indicate a sizeable voluntary reduction in debit card transactions in response to a positive infection shock. KOF Working Papers, 494

In this study, with the aim of revitalizing the region, how children's consciousness changes through activities such as children covering the region, writing articles, and expressing their thoughts from the perspective of Regional Journalism. From the perspective of Regional Journalism, we examined the activities of the Kids Reporter Club and clarified its　significance.As a research method, we compared and examined the results of the questionnaire survey in 2020, which was the interview in COVID-19 calamity, and the questionnaire survey in 2017 before COVID-19 calamity, and analyzed how much it is thought that the qualities and abilities as Regional Journalism were acquired.It can be considered that the element of Regional Journalism is included in the element of Citizenship by the activities of the Kids Reporter Club. It was found that the activities of the Kids Reporter Club, which incorporates the perspective of Regional Journalism, contribute to the development of Citizenship in the interview activities in COVID-19.From the perspective of Regional Journalism, we hope that children's reporters will disseminate information about the region, creating sympathy for many people in the region, and thereby revitalizing the region. However, the relationship between the activities of the children's press club and the revitalization of the community has not been verified. In the future, I would like to examine what kind of impact the activities of the Children's Press Club have on the community and whether they can.