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  • Open Access English
    Authors: 
    Ivano Baronchelli; G. Rodighiero; Harry I. Teplitz; Claudia Scarlata; Alberto Franceschini; S. Berta; Laia Barrufet; Mattia Vaccari; Matteo Bonato; Laure Ciesla; +15 more
    Publisher: HAL CCSD
    Countries: France, Italy, United States
    Project: EC | HELP (607254)

    For a sample of star forming galaxies in the redshift interval 0.15$<$z$<$0.3, we study how both the relative strength of the AGN infra-red emission, compared to that due to the star formation (SF), and the numerical fraction of AGNs, change as a function of the total stellar mass of the hosting galaxy group (M$^{*}_{\mathrm{group}}$), between $10^{10.25}$ and $10^{11.9}$M$_{\odot}$. Using a multi-component SED fitting analysis, we separate the contribution of stars, AGN torus and star formation to the total emission at different wavelengths. This technique is applied to a new multi-wavelength data-set in the SIMES field (23 not redundant photometric bands), spanning the wavelength range from the UV (GALEX) to the far-IR (Herschel) and including crucial AKARI and WISE mid-IR observations (4.5 \mu m$<\lambda<$24 \mu m), where the BH thermal emission is stronger. This new photometric catalog, that includes our best photo-z estimates, is released through the NASA/IPAC Infrared Science Archive (IRSA). Groups are identified through a friends of friends algorithm ($\sim$62% purity, $\sim$51% completeness). We identified a total of 45 galaxies requiring an AGN emission component, 35 of which in groups and 10 in the field. We find BHAR$\propto ($M$^{*}_{\mathrm{group}})^{1.21\pm0.27}$ and (BHAR/SFR)$\propto ($M$^{*}_{\mathrm{group}})^{1.04\pm0.24}$ while, in the same range of M$^{*}_{\mathrm{group}}$, we do not observe any sensible change in the numerical fraction of AGNs. Our results indicate that the nuclear activity (i.e. the BHAR and the BHAR/SFR ratio) is enhanced when galaxies are located in more massive and richer groups. Comment: 31 pages, 23 figures

  • Open Access
    Authors: 
    Alessandro Poli; Giulia Ramazzotti; Alessandro Matteucci; Lucia Manzoli; Annalisa Lonetti; Pann-Ghill Suh; James A. McCubrey; Lucio Cocco;
    Country: Italy

    // Alessandro Poli 1 , Giulia Ramazzotti 1 , Alessandro Matteucci 2 , Lucia Manzoli 1 , Annalisa Lonetti 1 , Pann-Ghill Suh 3 , James A. McCubrey 4 , Lucio Cocco 1 1 Cell Signaling Laboratory, Department of Biomedical Sciences, University of Bologna, Bologna, Italy 2 CNR-National Research Council of Italy, Institute of Molecular Genetics, Bologna, Italy 3 School of Nano-Biotechnology and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea 4 Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA Correspondence to: Lucio Cocco, e-mail: lucio.cocco@unibo.it. Keywords: PKC, Cyclin, Cell Cycle, PLC, DAG, nuclei Received: September 02, 2014 Accepted: October 06, 2014 Published: October 24, 2014 ABSTRACT Through the years, different studies showed the involvement of Protein Kinase C (PKC) in cell cycle control, in particular during G1/S transition. Little is known about their role at G2/M checkpoint. In this study, using K562 human erythroleukemia cell line, we found a novel and specific mechanism through which the conventional isoform PKCα positively affects Cyclin B1 modulating G2/M progression of cell cycle. Since the kinase activity of this PKC isoform was not necessary in this process, we demonstrated that PKCα, physically interacting with Cyclin B1, avoided its degradation and stimulated its nuclear import at mitosis. Moreover, the process resulted to be strictly connected with the increase in nuclear diacylglycerol levels (DAG) at G2/M checkpoint, due to the activity of nuclear Phospholipase C β1 (PLCβ1), the only PLC isoform mainly localized in the nucleus of K562 cells. Taken together, our findings indicated a novel DAG dependent mechanism able to regulate the G2/M progression of the cell cycle.

  • Open Access
    Authors: 
    Davide Tamburro; Sinisa Bratulic; Souad Abou Shameh; Nikul K. Soni; Andrea Bacconi; Francesca Maccari; Fabio Galeotti; Karin Mattsson; Nicola Volpi; Jens Nielsen; +1 more
    Publisher: Elsevier BV
    Project: EC | AURORAX (849251)

    AbstractGlycosaminoglycans (GAGs) are long linear sulfated polysaccharides implicated in processes linked to disease development such as mucopolysaccharidosis, respiratory failure, cancer, and viral infections, thereby serving as potential biomarkers. A successful clinical translation of GAGs as biomarkers depends on the availability of standardized GAG measurements. However, owing to the analytical complexity associated with the quantification of GAG concentration and structural composition, a standardized method to simultaneously measure multiple GAGs is missing. In this study, we sought to characterize the analytical performance of a ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UHPLC-MS/MS)-based kit for the quantification of 17 GAG disaccharides. The kit showed acceptable linearity, selectivity and specificity, accuracy and precision, and analyte stability in the absolute quantification of 15 GAG disaccharides. In native human samples, here using urine as a reference matrix, the analytical performance of the kit was acceptable for the quantification of CS disaccharides. Intra- and inter-laboratory tests performed in an external laboratory demonstrated robust reproducibility of GAG measurements showing that the kit was acceptably standardized. In conclusion, these results indicated that the UHPLC-MS/MS kit was standardized for the simultaneous measurement of GAG disaccharides allowing for comparability of measurements and enabling translational research.SummaryAnalytical performance of a kit for standardized GAG measurements, based on an established UHPLC-MS/MS method

  • Open Access
    Authors: 
    Luca Murru; Elena Vezzoli; Anna Longatti; Luisa Ponzoni; Andrea Falqui; Alessandra Folci; Edoardo Moretto; Veronica Bianchi; Daniela Braida; Mariaelvina Sala; +4 more
    Publisher: Oxford University Press (OUP)

    Abstract Intellectual disability affects 2–3% of the world's population and typically begins during childhood, causing impairments in social skills and cognitive abilities. Mutations in the TM4SF2 gene, which encodes the TSPAN7 protein, cause a severe form of intellectual disability, and currently, no therapy is able to ameliorate this cognitive impairment. We previously reported that, in cultured neurons, shRNA-mediated down-regulation of TSPAN7 affects AMPAR trafficking by enhancing PICK1–GluA2 interaction, thereby increasing the intracellular retention of AMPAR. Here, we found that loss of TSPAN7 function in mice causes alterations in hippocampal excitatory synapse structure and functionality as well as cognitive impairment. These changes occurred along with alterations in AMPAR expression levels. We also found that interfering with PICK1–GluA2 binding restored synaptic function in Tm4sf2−/y mice. Moreover, potentiation of AMPAR activity via the administration of the ampakine CX516 reverted the neurological phenotype observed in Tm4sf2−/y mice, suggesting that pharmacological modulation of AMPAR may represent a new approach for treating patients affected by TM4SF2 mutations and intellectual disability.

  • Closed Access English
    Authors: 
    A. Pileri; C. Agostinelli; A. Barisani; A. Patrizi; M. La Placa;
    Country: Italy

    not available

  • Open Access English
    Authors: 
    Camilla Dal Bosco; Davide Rossi; Andrea Brunetta; Antonio Bettero;
    Publisher: MDPI AG

    To evaluate the influence of the skin aging critical level on the adult skin epidermal functional state, an improved analytical method based on the skin surface energetic measurement (TVS modeling) was developed. Tenskinmetric measurements were carried out non-invasively in controlled conditions by contact angle method using only a water-drop as reference standard liquid. Adult skin was monitored by TVS Observatory according to a specific and controlled thermal protocol (Camianta protocol) in use at the interconnected “Mamma Margherita Terme spa” of Terme Euganee. From June to November 2013, the surface free energy and the epidermal hydration level of adult skin were evaluated on arrival of 265 male and 569 female adult volunteers (51–90 years of age) and when they departed 2 weeks later. Sensitive measurements were carried out at 0.1 mN/m. High test compliance was obtained (93.2% of all guests). Very interesting results are obtained. The high sensitivity and discrimination power of tenskinmetry combined with a thermal Camianta protocol demonstrate the possibility to evaluate at baseline level the surface energetic changes and the skin reactivity which occurs on adult skin.

  • Open Access English
    Authors: 
    Bolotta, A.; Pini, A.; Abruzzo, P. M.; Ghezzo, A.; Modesti, A.; Gamberi, T.; Ferreri, C.; Bugamelli, F.; Fortuna, F.; Vertuani, S.; +3 more
    Country: Italy
  • Open Access English
    Authors: 
    Emanuele Schimmenti; Enrico Viola; Cassandra Funsten; Valeria Borsellino;
    Publisher: MDPI AG
    Country: Italy

    This study attempts to measure the capacity of the EU geographical certification scheme to positively influence the price of certified products and the incomes of their producers. A comparison of the economic results of two cheese-producing dairy farm enterprises with different business strategies and locations within the Sicilian hinterlands is performed in order to determine the transformation value of each dairy’s sheep milk into pecorino cheese (with and without the Protected Designation of Origin, or PDO, certification) and the related joint products (ricotta). The economic convenience of the total transformation of sheep milk into Pecorino Siciliano PDO and ricotta is also appraised. The results suggest that producing and commercializing Pecorino Siciliano PDO is a promising strategy for differentiating and promoting dairy farm products and improving the financial performance of producers, with foreseeable positive repercussions in the socioeconomically less favored rural areas where they are located.

  • Restricted
    Authors: 
    Gabriele Di Blasio; Carlo Beatrice; Roberto Ianniello; Francesco Concetto Pesce; Alberto Vassallo; Giacomo Belgiorno; Giovanni Avolio;
    Publisher: SAE International
    Country: Italy

    The introduction of new light-duty vehicle emission limits to comply under real driving conditions (RDE) is pushing the diesel engine manufacturers to identify and improve the technologies and strategies for further emission reduction. The latest technology advancements on the after-treatment systems have permitted to achieve very low emission conformity factors over the RDE, and therefore, the biggest challenge of the diesel engine development is maintaining its competitiveness in the trade-off "CO2-system cost" in comparison to other propulsion systems. In this regard, diesel engines can continue to play an important role, in the short-medium term, to enable cost-effective compliance of CO2-fleet emission targets, either in conventional or hybrid propulsion systems configuration. This is especially true for large-size cars, SUVs and light commercial vehicles. In this framework, a comprehensive approach covering the whole powertrain is of primary importance in order to simultaneously meet the performance, efficiency, noise and emission targets, and therefore, further development of the combustion system design and injection system represent important levers for additional improvements. For this purpose, a dedicated 0.5 dm3 single-cylinder engine has been developed and equipped with, a state-of-the-art Euro 6 combustion system, and an advanced common rail fuel injection system (FIS) offering higher flexibility in terms of injection strategy and higher quantity accuracy. Three injector nozzles with different hydraulic flow rates (HF) have been selected and employed for the overall combustion process optimization. The optimization has been performed by means of an extensive DoE-based test campaign in which the engine and FIS operating parameters have been parametrized with the aim to carry out a proper combination in terms of HF and injection strategy. The results at partial load conditions evidence significant advantages in applying an advanced injection pattern, while the HF reduction can significantly improve the smoke emission and combustion noise without fuel consumption penalties. Therefore, a proper combination and optimization of the HF and injection strategy can provide low noise and engine-out smoke while maintaining the rated power performance targets.

  • Publication . Article . 2014
    Open Access Italian
    Authors: 
    Gestri, Marco;
    Country: Italy
Advanced search in
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912,520 Research products, page 1 of 91,252
  • Open Access English
    Authors: 
    Ivano Baronchelli; G. Rodighiero; Harry I. Teplitz; Claudia Scarlata; Alberto Franceschini; S. Berta; Laia Barrufet; Mattia Vaccari; Matteo Bonato; Laure Ciesla; +15 more
    Publisher: HAL CCSD
    Countries: France, Italy, United States
    Project: EC | HELP (607254)

    For a sample of star forming galaxies in the redshift interval 0.15$<$z$<$0.3, we study how both the relative strength of the AGN infra-red emission, compared to that due to the star formation (SF), and the numerical fraction of AGNs, change as a function of the total stellar mass of the hosting galaxy group (M$^{*}_{\mathrm{group}}$), between $10^{10.25}$ and $10^{11.9}$M$_{\odot}$. Using a multi-component SED fitting analysis, we separate the contribution of stars, AGN torus and star formation to the total emission at different wavelengths. This technique is applied to a new multi-wavelength data-set in the SIMES field (23 not redundant photometric bands), spanning the wavelength range from the UV (GALEX) to the far-IR (Herschel) and including crucial AKARI and WISE mid-IR observations (4.5 \mu m$<\lambda<$24 \mu m), where the BH thermal emission is stronger. This new photometric catalog, that includes our best photo-z estimates, is released through the NASA/IPAC Infrared Science Archive (IRSA). Groups are identified through a friends of friends algorithm ($\sim$62% purity, $\sim$51% completeness). We identified a total of 45 galaxies requiring an AGN emission component, 35 of which in groups and 10 in the field. We find BHAR$\propto ($M$^{*}_{\mathrm{group}})^{1.21\pm0.27}$ and (BHAR/SFR)$\propto ($M$^{*}_{\mathrm{group}})^{1.04\pm0.24}$ while, in the same range of M$^{*}_{\mathrm{group}}$, we do not observe any sensible change in the numerical fraction of AGNs. Our results indicate that the nuclear activity (i.e. the BHAR and the BHAR/SFR ratio) is enhanced when galaxies are located in more massive and richer groups. Comment: 31 pages, 23 figures

  • Open Access
    Authors: 
    Alessandro Poli; Giulia Ramazzotti; Alessandro Matteucci; Lucia Manzoli; Annalisa Lonetti; Pann-Ghill Suh; James A. McCubrey; Lucio Cocco;
    Country: Italy

    // Alessandro Poli 1 , Giulia Ramazzotti 1 , Alessandro Matteucci 2 , Lucia Manzoli 1 , Annalisa Lonetti 1 , Pann-Ghill Suh 3 , James A. McCubrey 4 , Lucio Cocco 1 1 Cell Signaling Laboratory, Department of Biomedical Sciences, University of Bologna, Bologna, Italy 2 CNR-National Research Council of Italy, Institute of Molecular Genetics, Bologna, Italy 3 School of Nano-Biotechnology and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea 4 Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA Correspondence to: Lucio Cocco, e-mail: lucio.cocco@unibo.it. Keywords: PKC, Cyclin, Cell Cycle, PLC, DAG, nuclei Received: September 02, 2014 Accepted: October 06, 2014 Published: October 24, 2014 ABSTRACT Through the years, different studies showed the involvement of Protein Kinase C (PKC) in cell cycle control, in particular during G1/S transition. Little is known about their role at G2/M checkpoint. In this study, using K562 human erythroleukemia cell line, we found a novel and specific mechanism through which the conventional isoform PKCα positively affects Cyclin B1 modulating G2/M progression of cell cycle. Since the kinase activity of this PKC isoform was not necessary in this process, we demonstrated that PKCα, physically interacting with Cyclin B1, avoided its degradation and stimulated its nuclear import at mitosis. Moreover, the process resulted to be strictly connected with the increase in nuclear diacylglycerol levels (DAG) at G2/M checkpoint, due to the activity of nuclear Phospholipase C β1 (PLCβ1), the only PLC isoform mainly localized in the nucleus of K562 cells. Taken together, our findings indicated a novel DAG dependent mechanism able to regulate the G2/M progression of the cell cycle.

  • Open Access
    Authors: 
    Davide Tamburro; Sinisa Bratulic; Souad Abou Shameh; Nikul K. Soni; Andrea Bacconi; Francesca Maccari; Fabio Galeotti; Karin Mattsson; Nicola Volpi; Jens Nielsen; +1 more
    Publisher: Elsevier BV
    Project: EC | AURORAX (849251)

    AbstractGlycosaminoglycans (GAGs) are long linear sulfated polysaccharides implicated in processes linked to disease development such as mucopolysaccharidosis, respiratory failure, cancer, and viral infections, thereby serving as potential biomarkers. A successful clinical translation of GAGs as biomarkers depends on the availability of standardized GAG measurements. However, owing to the analytical complexity associated with the quantification of GAG concentration and structural composition, a standardized method to simultaneously measure multiple GAGs is missing. In this study, we sought to characterize the analytical performance of a ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UHPLC-MS/MS)-based kit for the quantification of 17 GAG disaccharides. The kit showed acceptable linearity, selectivity and specificity, accuracy and precision, and analyte stability in the absolute quantification of 15 GAG disaccharides. In native human samples, here using urine as a reference matrix, the analytical performance of the kit was acceptable for the quantification of CS disaccharides. Intra- and inter-laboratory tests performed in an external laboratory demonstrated robust reproducibility of GAG measurements showing that the kit was acceptably standardized. In conclusion, these results indicated that the UHPLC-MS/MS kit was standardized for the simultaneous measurement of GAG disaccharides allowing for comparability of measurements and enabling translational research.SummaryAnalytical performance of a kit for standardized GAG measurements, based on an established UHPLC-MS/MS method

  • Open Access
    Authors: 
    Luca Murru; Elena Vezzoli; Anna Longatti; Luisa Ponzoni; Andrea Falqui; Alessandra Folci; Edoardo Moretto; Veronica Bianchi; Daniela Braida; Mariaelvina Sala; +4 more
    Publisher: Oxford University Press (OUP)

    Abstract Intellectual disability affects 2–3% of the world's population and typically begins during childhood, causing impairments in social skills and cognitive abilities. Mutations in the TM4SF2 gene, which encodes the TSPAN7 protein, cause a severe form of intellectual disability, and currently, no therapy is able to ameliorate this cognitive impairment. We previously reported that, in cultured neurons, shRNA-mediated down-regulation of TSPAN7 affects AMPAR trafficking by enhancing PICK1–GluA2 interaction, thereby increasing the intracellular retention of AMPAR. Here, we found that loss of TSPAN7 function in mice causes alterations in hippocampal excitatory synapse structure and functionality as well as cognitive impairment. These changes occurred along with alterations in AMPAR expression levels. We also found that interfering with PICK1–GluA2 binding restored synaptic function in Tm4sf2−/y mice. Moreover, potentiation of AMPAR activity via the administration of the ampakine CX516 reverted the neurological phenotype observed in Tm4sf2−/y mice, suggesting that pharmacological modulation of AMPAR may represent a new approach for treating patients affected by TM4SF2 mutations and intellectual disability.

  • Closed Access English
    Authors: 
    A. Pileri; C. Agostinelli; A. Barisani; A. Patrizi; M. La Placa;
    Country: Italy

    not available

  • Open Access English
    Authors: 
    Camilla Dal Bosco; Davide Rossi; Andrea Brunetta; Antonio Bettero;
    Publisher: MDPI AG

    To evaluate the influence of the skin aging critical level on the adult skin epidermal functional state, an improved analytical method based on the skin surface energetic measurement (TVS modeling) was developed. Tenskinmetric measurements were carried out non-invasively in controlled conditions by contact angle method using only a water-drop as reference standard liquid. Adult skin was monitored by TVS Observatory according to a specific and controlled thermal protocol (Camianta protocol) in use at the interconnected “Mamma Margherita Terme spa” of Terme Euganee. From June to November 2013, the surface free energy and the epidermal hydration level of adult skin were evaluated on arrival of 265 male and 569 female adult volunteers (51–90 years of age) and when they departed 2 weeks later. Sensitive measurements were carried out at 0.1 mN/m. High test compliance was obtained (93.2% of all guests). Very interesting results are obtained. The high sensitivity and discrimination power of tenskinmetry combined with a thermal Camianta protocol demonstrate the possibility to evaluate at baseline level the surface energetic changes and the skin reactivity which occurs on adult skin.

  • Open Access English
    Authors: 
    Bolotta, A.; Pini, A.; Abruzzo, P. M.; Ghezzo, A.; Modesti, A.; Gamberi, T.; Ferreri, C.; Bugamelli, F.; Fortuna, F.; Vertuani, S.; +3 more
    Country: Italy
  • Open Access English
    Authors: 
    Emanuele Schimmenti; Enrico Viola; Cassandra Funsten; Valeria Borsellino;
    Publisher: MDPI AG
    Country: Italy

    This study attempts to measure the capacity of the EU geographical certification scheme to positively influence the price of certified products and the incomes of their producers. A comparison of the economic results of two cheese-producing dairy farm enterprises with different business strategies and locations within the Sicilian hinterlands is performed in order to determine the transformation value of each dairy’s sheep milk into pecorino cheese (with and without the Protected Designation of Origin, or PDO, certification) and the related joint products (ricotta). The economic convenience of the total transformation of sheep milk into Pecorino Siciliano PDO and ricotta is also appraised. The results suggest that producing and commercializing Pecorino Siciliano PDO is a promising strategy for differentiating and promoting dairy farm products and improving the financial performance of producers, with foreseeable positive repercussions in the socioeconomically less favored rural areas where they are located.

  • Restricted
    Authors: 
    Gabriele Di Blasio; Carlo Beatrice; Roberto Ianniello; Francesco Concetto Pesce; Alberto Vassallo; Giacomo Belgiorno; Giovanni Avolio;
    Publisher: SAE International
    Country: Italy

    The introduction of new light-duty vehicle emission limits to comply under real driving conditions (RDE) is pushing the diesel engine manufacturers to identify and improve the technologies and strategies for further emission reduction. The latest technology advancements on the after-treatment systems have permitted to achieve very low emission conformity factors over the RDE, and therefore, the biggest challenge of the diesel engine development is maintaining its competitiveness in the trade-off "CO2-system cost" in comparison to other propulsion systems. In this regard, diesel engines can continue to play an important role, in the short-medium term, to enable cost-effective compliance of CO2-fleet emission targets, either in conventional or hybrid propulsion systems configuration. This is especially true for large-size cars, SUVs and light commercial vehicles. In this framework, a comprehensive approach covering the whole powertrain is of primary importance in order to simultaneously meet the performance, efficiency, noise and emission targets, and therefore, further development of the combustion system design and injection system represent important levers for additional improvements. For this purpose, a dedicated 0.5 dm3 single-cylinder engine has been developed and equipped with, a state-of-the-art Euro 6 combustion system, and an advanced common rail fuel injection system (FIS) offering higher flexibility in terms of injection strategy and higher quantity accuracy. Three injector nozzles with different hydraulic flow rates (HF) have been selected and employed for the overall combustion process optimization. The optimization has been performed by means of an extensive DoE-based test campaign in which the engine and FIS operating parameters have been parametrized with the aim to carry out a proper combination in terms of HF and injection strategy. The results at partial load conditions evidence significant advantages in applying an advanced injection pattern, while the HF reduction can significantly improve the smoke emission and combustion noise without fuel consumption penalties. Therefore, a proper combination and optimization of the HF and injection strategy can provide low noise and engine-out smoke while maintaining the rated power performance targets.

  • Publication . Article . 2014
    Open Access Italian
    Authors: 
    Gestri, Marco;
    Country: Italy
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