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  • Publication . Part of book or chapter of book . 2019
    Closed Access
    Authors: 
    Dan E. Webster; Sandrine Roulland; James D. Phelan;
    Country: France
    Project: EC | LYMPHOSIGN (661066)

    Genome-wide screens are a powerful technique to dissect the complex network of genes regulating diverse cellular phenotypes. The recent adaptation of the CRISPR-Cas9 system for genome engineering has revolutionized functional genomic screening. Here, we present protocols used to introduce Cas9 into human lymphoma cell lines, produce high-titer lentivirus of a genome-wide sgRNA library, transduce and culture cells during the screen, isolate genomic DNA, and prepare a custom library for next-generation sequencing. These protocols were tailored for loss-of-function CRISPR screens in human lymphoma cell lines but are highly amenable for other experimental purposes.

  • Open Access English
    Authors: 
    Martin, Adrian; Coolsaet, Brendan; Corbera, Esteve; Dawson, Neil; Fisher, Janet; Franks, Phil; Mertz, Ole; Pascual, Unai; Rasmussen, Laura,; Ryan, Casey;
    Publisher: Routledge
    Countries: France, United Kingdom

    Land use intensification is widely considered to be an essential strategy for achieving global goals to eliminate poverty and to avoid damaging losses of ecosystem services. This chapter investigates whether current land use intensification activities are achieving these twin goals. To do so, it reviews a body of academic literature that reports on case studies in which both social and ecological outcomes of intensification are reported. There are two main findings. First, there are relatively few cases in which land use intensification is clearly succeeding in these twinned objectives. There are many more cases in which, for example, short-term income or productivity gains from land use intensification are resulting in long-term diminution of biodiversity and ecosystem services. Studies with longer-term perspectives are already seeing how such trade-offs are leading to negative feedbacks for human wellbeing, especially for marginalised social groups. Secondly, we learn most from those studies that a) go beyond measuring production and income to measure multiple dimensions of wellbeing and ecosystem services, b) monitor dynamics of outcomes across longer time periods and across landscapes and c) disaggregate outcome measures to identify outcomes for different social groups.

  • Publication . Part of book or chapter of book . Preprint . Article . 2020
    Open Access English
    Authors: 
    Florian Bridoux; Maximilien Gadouleau; Guillaume Theyssier;
    Publisher: HAL CCSD
    Country: France

    Automata networks are mappings of the form \(f: Q^Z \rightarrow Q^Z\), where Q is a finite alphabet and Z is a set of entities; they generalise Cellular Automata and Boolean networks. An update schedule dictates when each entity updates its state according to its local function \(f_i: Q^Z \rightarrow Q\). One major question is to study the behaviour of a given automata networks under different update schedules. In this paper, we study automata networks that are invariant under many different update schedules. This gives rise to two definitions, locally commutative and globally commutative networks. We investigate the relation between commutativity and different forms of locality of update functions; one main conclusion is that globally commutative networks have strong dynamical properties, while locally commutative networks are much less constrained. We also give a complete classification of all globally commutative Boolean networks.

  • Closed Access
    Authors: 
    Erran Carmel; Steve Sawyer;
    Publisher: Springer International Publishing
    Country: France

    Part 6: The Dark Side of Digitalisation; International audience; We advance a structured view of the Futures of Work (FoW) using a futurist’s lens to advance two goals: advancing core dimensions to the FoW while outlining the Futurist’s approach to considering possible futures. Professional futurists point out that they do not predict the future, but rather, build a number of futures – in plural. These views of the futures are presented as scenarios to help decision makers consider alternatives and better understand interactions among the planning dimensions. The scenarios that drive planning are constructed by drawing on characteristics or dimensions that will shape our futures. It is these dimensions that we present here. We offer five foundational dimensions for the FoW, articulating them as opposing perspectives to frame the issue: (1) Virtuality versus Compressed working arrangements; (2) Atomistic work versus Holistic work; (3) Algorithmic versus Human decision-making; (4) Neoliberal capitalism versus Safety-net capitalism; (5) Übermensch versus Nihilists. We use these dimensions to provide scenarios to illustrate their use. We conclude by reflecting on the shock of the 2020 pandemic and the roles of firm size relative to the futures of work.

  • Closed Access English
    Authors: 
    Jean-Paul Carrière; Stuart Farthing;
    Publisher: HAL CCSD
    Country: France

    In this research, we investigated the environmental as well as social impacts of the regeneration of urban riverbanks and waterfronts, localities previously occupied by port-industrial activities established during the twentieth century or earlier, but more recently abandoned and occupied by informal housing and wasteland or brownfield land. The urban plans and strategies of metropolises as well as of medium-sized cities have become increasingly underpinned by the “paradigm of attractiveness,” in a context of globalization and competition between cities at an international scale. In order to become more attractive for investment, mainly foreign direct investment, cities need to improve their image and their international ranking. The recycling of the abandoned wasteland in these areas has become a policy priority. Under the banner of sustainable development, strategic plans in many cities throughout the world have thus promoted flagship projects for the regeneration of these waterfront areas, incorporating new environmental amenities.

  • Publication . Part of book or chapter of book . 2019
    Open Access English
    Authors: 
    Marco Buongiorno Nardelli;
    Publisher: HAL CCSD
    Country: France

    We present the API for MUSICNTWRK, a python library for pitch class set and rhythmic sequences classification and manipulation, the generation of networks in generalized music and sound spaces, deep learning algorithms for timbre recognition, and the sonification of arbitrary data. The software is freely available under GPL 3.0 and can be downloaded at www.musicntwrk.com or installed as a PyPi project (pip install musicntwrk).

  • Publication . Part of book or chapter of book . 2022
    French
    Authors: 
    Patte, Marie-France;
    Publisher: HAL CCSD
    Country: France

    la copie déposée est une première version de l'article paru en 2011. Cette publication fait suite au congrès 2005 de AAA à Washington session: "Endangered languages and Linguistic anthropology".; The Añun language (also known as "Parauhano") is still spoken by a few aged individuals, all speaking Spanish in everyday activities and living in lake-dwellings between Maracaibo and the Venezuelan border with Colombia. The territory of the Añun people, disputed ever since the first contacts with the Europeans, has progressively shrunk with the increasing importance of the oil industry. Their linguistic situation, reflected in their oral narratives, takes us back to the power relations experienced by the speakers. Besides providing the community with the widest range of documentation as possible, linguistic anthropology assumes a participative approach, which can help create a partnership between elder speakers and young members of the community, in order to preserve traditional knowledge and develop different aspects of the language and the culture. To be effective, the revitalization of the language should go together with territorial claims and identity recognition.

  • Publication . Conference object . Part of book or chapter of book . 2018
    Open Access English
    Authors: 
    Lucy Suchman;
    Publisher: HAL CCSD
    Country: France

    Part 1: Setting the Stage; International audience; This text is based on an invited address presented at IFIP 8.2 ‘Living with Monsters’ in San Francisco, CA, 11 December 2018. Taking the 200th anniversary of Mary Wollstonecraft Shelley’s Frankenstein as a starting place, I explore questions of autonomy and control with respect to human/technology relations. I consider the ambivalence of these agencies, and recent initiatives in science and technology studies and related fields to reconceptualize the problem as matters of relation and care. While embracing this turn, I reflect as well upon the ambivalences of relation and care, and the need to address the resilient politics of alterity in our figurations (and celebrations) of the monstrous.

  • Publication . Conference object . Article . Part of book or chapter of book . 2020
    Open Access English
    Authors: 
    Alexis Joly; Hervé Goëau; Stefan Kahl; Christophe Botella; Rafael Ruiz De Castaneda; Hervé Glotin; Elijah Cole; Julien Champ; Benjamin Deneu; Maximillien Servajean; +6 more
    Publisher: HAL CCSD
    Country: France
    Project: NSF | Graduate Research Fellows... (1745301), EC | COS4CLOUD (863463), ANR | SMILES (ANR-18-CE40-0014)

    International audience; Building accurate knowledge of the identity, the geographic distribution and the evolution of species is essential for the sustainable development of humanity, as well as for biodiversity conservation. However, the difficulty of identifying plants and animals in the field is hindering the aggregation of new data and knowledge. Identifying and naming living plants or animals is almost impossible for the general public and is often difficult even for professionals and naturalists. Bridging this gap is a key step towards enabling effective biodiversity monitoring systems. The LifeCLEF campaign, presented in this paper, has been promoting and evaluating advances in this domain since 2011. The 2020 edition proposes four data-oriented challenges related to the identification and prediction of biodiversity: (i) PlantCLEF: cross-domain plant identification based on herbarium sheets, (ii) BirdCLEF: bird species recognition in audio soundscapes, (iii) GeoLifeCLEF: location-based prediction of species based on environmental and occurrence data, and (iv) SnakeCLEF: image-based snake identification.

  • Publication . Part of book or chapter of book . 2019
    Open Access
    Authors: 
    Pierre Drap; Odile Papini; Djamal Merad; Jérôme Pasquet; Jean-Philip Royer; Mohamad Motasem Nawaf; Mauro Saccone; Mohamed Ben Ellefi; Bertrand Chemisky; Julien Seinturier; +3 more
    Publisher: Springer International Publishing
    Country: France
    Project: ANR | GROPLAN (ANR-13-CORD-0014)

    International audience; This chapter introduces several state of the art techniques that could help to make deep underwater archaeological photogrammetric surveys easier, faster, more accurate, and to provide more visually appealing representations in 2D and 3D for both experts and public. We detail how the 3D captured data is analysed and then represented using ontologies, and how this facilitates interdisciplinary interpretation and cooperation. Towards more automation, we present a new method that adopts a deep learning approach for the detection and the recognition of objects of interest, amphorae for example. In order to provide more readable, direct and clearer illustrations, we describe several techniques that generate different styles of sketches out of orthophotos developed using neural networks. In the same direction, we present the Non-Photorealistic Rendering (NPR) technique, which converts a 3D model into a more readable 2D representation that is more useful to communicate and simplifies the identification of objects of interest. Regarding public dissemination, we demonstrate how recent advances in virtual reality to provide an accurate, high resolution, amusing and appropriate visualization tool that offers the public the possibility to ‘visit’ an unreachable archaeological site. Finally, we conclude by introducing the plenoptic approach, a new promising technology that can change the future of the photogrammetry by making it easier and less time consuming and that allows a user to create a 3D model using only one camera shot. Here, we introduce the concepts, the developing process, and some results, which we obtained with underwater imaging.

Advanced search in
Research products
arrow_drop_down
Searching FieldsTerms
Any field
arrow_drop_down
includes
arrow_drop_down
Include:
407 Research products, page 1 of 41
  • Publication . Part of book or chapter of book . 2019
    Closed Access
    Authors: 
    Dan E. Webster; Sandrine Roulland; James D. Phelan;
    Country: France
    Project: EC | LYMPHOSIGN (661066)

    Genome-wide screens are a powerful technique to dissect the complex network of genes regulating diverse cellular phenotypes. The recent adaptation of the CRISPR-Cas9 system for genome engineering has revolutionized functional genomic screening. Here, we present protocols used to introduce Cas9 into human lymphoma cell lines, produce high-titer lentivirus of a genome-wide sgRNA library, transduce and culture cells during the screen, isolate genomic DNA, and prepare a custom library for next-generation sequencing. These protocols were tailored for loss-of-function CRISPR screens in human lymphoma cell lines but are highly amenable for other experimental purposes.

  • Open Access English
    Authors: 
    Martin, Adrian; Coolsaet, Brendan; Corbera, Esteve; Dawson, Neil; Fisher, Janet; Franks, Phil; Mertz, Ole; Pascual, Unai; Rasmussen, Laura,; Ryan, Casey;
    Publisher: Routledge
    Countries: France, United Kingdom

    Land use intensification is widely considered to be an essential strategy for achieving global goals to eliminate poverty and to avoid damaging losses of ecosystem services. This chapter investigates whether current land use intensification activities are achieving these twin goals. To do so, it reviews a body of academic literature that reports on case studies in which both social and ecological outcomes of intensification are reported. There are two main findings. First, there are relatively few cases in which land use intensification is clearly succeeding in these twinned objectives. There are many more cases in which, for example, short-term income or productivity gains from land use intensification are resulting in long-term diminution of biodiversity and ecosystem services. Studies with longer-term perspectives are already seeing how such trade-offs are leading to negative feedbacks for human wellbeing, especially for marginalised social groups. Secondly, we learn most from those studies that a) go beyond measuring production and income to measure multiple dimensions of wellbeing and ecosystem services, b) monitor dynamics of outcomes across longer time periods and across landscapes and c) disaggregate outcome measures to identify outcomes for different social groups.

  • Publication . Part of book or chapter of book . Preprint . Article . 2020
    Open Access English
    Authors: 
    Florian Bridoux; Maximilien Gadouleau; Guillaume Theyssier;
    Publisher: HAL CCSD
    Country: France

    Automata networks are mappings of the form \(f: Q^Z \rightarrow Q^Z\), where Q is a finite alphabet and Z is a set of entities; they generalise Cellular Automata and Boolean networks. An update schedule dictates when each entity updates its state according to its local function \(f_i: Q^Z \rightarrow Q\). One major question is to study the behaviour of a given automata networks under different update schedules. In this paper, we study automata networks that are invariant under many different update schedules. This gives rise to two definitions, locally commutative and globally commutative networks. We investigate the relation between commutativity and different forms of locality of update functions; one main conclusion is that globally commutative networks have strong dynamical properties, while locally commutative networks are much less constrained. We also give a complete classification of all globally commutative Boolean networks.

  • Closed Access
    Authors: 
    Erran Carmel; Steve Sawyer;
    Publisher: Springer International Publishing
    Country: France

    Part 6: The Dark Side of Digitalisation; International audience; We advance a structured view of the Futures of Work (FoW) using a futurist’s lens to advance two goals: advancing core dimensions to the FoW while outlining the Futurist’s approach to considering possible futures. Professional futurists point out that they do not predict the future, but rather, build a number of futures – in plural. These views of the futures are presented as scenarios to help decision makers consider alternatives and better understand interactions among the planning dimensions. The scenarios that drive planning are constructed by drawing on characteristics or dimensions that will shape our futures. It is these dimensions that we present here. We offer five foundational dimensions for the FoW, articulating them as opposing perspectives to frame the issue: (1) Virtuality versus Compressed working arrangements; (2) Atomistic work versus Holistic work; (3) Algorithmic versus Human decision-making; (4) Neoliberal capitalism versus Safety-net capitalism; (5) Übermensch versus Nihilists. We use these dimensions to provide scenarios to illustrate their use. We conclude by reflecting on the shock of the 2020 pandemic and the roles of firm size relative to the futures of work.

  • Closed Access English
    Authors: 
    Jean-Paul Carrière; Stuart Farthing;
    Publisher: HAL CCSD
    Country: France

    In this research, we investigated the environmental as well as social impacts of the regeneration of urban riverbanks and waterfronts, localities previously occupied by port-industrial activities established during the twentieth century or earlier, but more recently abandoned and occupied by informal housing and wasteland or brownfield land. The urban plans and strategies of metropolises as well as of medium-sized cities have become increasingly underpinned by the “paradigm of attractiveness,” in a context of globalization and competition between cities at an international scale. In order to become more attractive for investment, mainly foreign direct investment, cities need to improve their image and their international ranking. The recycling of the abandoned wasteland in these areas has become a policy priority. Under the banner of sustainable development, strategic plans in many cities throughout the world have thus promoted flagship projects for the regeneration of these waterfront areas, incorporating new environmental amenities.

  • Publication . Part of book or chapter of book . 2019
    Open Access English
    Authors: 
    Marco Buongiorno Nardelli;
    Publisher: HAL CCSD
    Country: France

    We present the API for MUSICNTWRK, a python library for pitch class set and rhythmic sequences classification and manipulation, the generation of networks in generalized music and sound spaces, deep learning algorithms for timbre recognition, and the sonification of arbitrary data. The software is freely available under GPL 3.0 and can be downloaded at www.musicntwrk.com or installed as a PyPi project (pip install musicntwrk).

  • Publication . Part of book or chapter of book . 2022
    French
    Authors: 
    Patte, Marie-France;
    Publisher: HAL CCSD
    Country: France

    la copie déposée est une première version de l'article paru en 2011. Cette publication fait suite au congrès 2005 de AAA à Washington session: "Endangered languages and Linguistic anthropology".; The Añun language (also known as "Parauhano") is still spoken by a few aged individuals, all speaking Spanish in everyday activities and living in lake-dwellings between Maracaibo and the Venezuelan border with Colombia. The territory of the Añun people, disputed ever since the first contacts with the Europeans, has progressively shrunk with the increasing importance of the oil industry. Their linguistic situation, reflected in their oral narratives, takes us back to the power relations experienced by the speakers. Besides providing the community with the widest range of documentation as possible, linguistic anthropology assumes a participative approach, which can help create a partnership between elder speakers and young members of the community, in order to preserve traditional knowledge and develop different aspects of the language and the culture. To be effective, the revitalization of the language should go together with territorial claims and identity recognition.

  • Publication . Conference object . Part of book or chapter of book . 2018
    Open Access English
    Authors: 
    Lucy Suchman;
    Publisher: HAL CCSD
    Country: France

    Part 1: Setting the Stage; International audience; This text is based on an invited address presented at IFIP 8.2 ‘Living with Monsters’ in San Francisco, CA, 11 December 2018. Taking the 200th anniversary of Mary Wollstonecraft Shelley’s Frankenstein as a starting place, I explore questions of autonomy and control with respect to human/technology relations. I consider the ambivalence of these agencies, and recent initiatives in science and technology studies and related fields to reconceptualize the problem as matters of relation and care. While embracing this turn, I reflect as well upon the ambivalences of relation and care, and the need to address the resilient politics of alterity in our figurations (and celebrations) of the monstrous.

  • Publication . Conference object . Article . Part of book or chapter of book . 2020
    Open Access English
    Authors: 
    Alexis Joly; Hervé Goëau; Stefan Kahl; Christophe Botella; Rafael Ruiz De Castaneda; Hervé Glotin; Elijah Cole; Julien Champ; Benjamin Deneu; Maximillien Servajean; +6 more
    Publisher: HAL CCSD
    Country: France
    Project: NSF | Graduate Research Fellows... (1745301), EC | COS4CLOUD (863463), ANR | SMILES (ANR-18-CE40-0014)

    International audience; Building accurate knowledge of the identity, the geographic distribution and the evolution of species is essential for the sustainable development of humanity, as well as for biodiversity conservation. However, the difficulty of identifying plants and animals in the field is hindering the aggregation of new data and knowledge. Identifying and naming living plants or animals is almost impossible for the general public and is often difficult even for professionals and naturalists. Bridging this gap is a key step towards enabling effective biodiversity monitoring systems. The LifeCLEF campaign, presented in this paper, has been promoting and evaluating advances in this domain since 2011. The 2020 edition proposes four data-oriented challenges related to the identification and prediction of biodiversity: (i) PlantCLEF: cross-domain plant identification based on herbarium sheets, (ii) BirdCLEF: bird species recognition in audio soundscapes, (iii) GeoLifeCLEF: location-based prediction of species based on environmental and occurrence data, and (iv) SnakeCLEF: image-based snake identification.

  • Publication . Part of book or chapter of book . 2019
    Open Access
    Authors: 
    Pierre Drap; Odile Papini; Djamal Merad; Jérôme Pasquet; Jean-Philip Royer; Mohamad Motasem Nawaf; Mauro Saccone; Mohamed Ben Ellefi; Bertrand Chemisky; Julien Seinturier; +3 more
    Publisher: Springer International Publishing
    Country: France
    Project: ANR | GROPLAN (ANR-13-CORD-0014)

    International audience; This chapter introduces several state of the art techniques that could help to make deep underwater archaeological photogrammetric surveys easier, faster, more accurate, and to provide more visually appealing representations in 2D and 3D for both experts and public. We detail how the 3D captured data is analysed and then represented using ontologies, and how this facilitates interdisciplinary interpretation and cooperation. Towards more automation, we present a new method that adopts a deep learning approach for the detection and the recognition of objects of interest, amphorae for example. In order to provide more readable, direct and clearer illustrations, we describe several techniques that generate different styles of sketches out of orthophotos developed using neural networks. In the same direction, we present the Non-Photorealistic Rendering (NPR) technique, which converts a 3D model into a more readable 2D representation that is more useful to communicate and simplifies the identification of objects of interest. Regarding public dissemination, we demonstrate how recent advances in virtual reality to provide an accurate, high resolution, amusing and appropriate visualization tool that offers the public the possibility to ‘visit’ an unreachable archaeological site. Finally, we conclude by introducing the plenoptic approach, a new promising technology that can change the future of the photogrammetry by making it easier and less time consuming and that allows a user to create a 3D model using only one camera shot. Here, we introduce the concepts, the developing process, and some results, which we obtained with underwater imaging.

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