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ProbeSeq results as received from the HOMINGS core facility. The first worksheet provides read count summaries for each sample. The second worksheet lists read counts for all HOMINGS probes (including those not detected) for each sample. The third worksheet presents the read counts as a percent of total reads for each sample, and the fourth worksheet summarizes results for each sample as stacked column plots.

Simulation of a black-hole binary system evolved by the SpEC code.

Additional file 6: Figure S1. Cumulative OTU-ranked abundance curves. Note the much larger OTU diversity in densely colonized JP07 and FL02 vs. lightly colonized microfloras FL01 and GM14. Cumulative abundances values below 80Â % not displayed for figure succinctness.

We sought to explain patterns in parasitism frequency of two specialist herbivores (Geometridae) by investigating the influence of plant diet as a source of variation in immune response variables important for defense against parasitism. Field collected caterpillars (Eois apyraria and Eois nympha) were assigned to one of two species in the plant genus Piper (Piperaceae): 1) a host species with high diversity of defensive chemistry, P. cenocladum C.DC., or 2) a host species with lower investment in chemical defense, P. imperiale C.DC. Hemolymph was extracted from fifth instar larvae, and immune strength measured unidimensionally using a phenoloxidase (PO) enzyme assay. Parasitism data came from 19 years of accumulated host plant-caterpillar-parasitoid associations from a long-term rearing project at La Selva Biological Station in Costa Rica, where the experiment took place. We found that immunity was significantly weakened when caterpillars were reared on the host plant with higher phytochemical diversity (P. cenocladum). Moreover, host plants inducing a weak immune response hosted caterpillars with higher parasitism rates. We conclude that patterns in parasitism frequency can be partially explained by cascading effects of host plant traits. Eois immune dataThe file contains immune response data on two species of Eois caterpillars feeding on different Piper species.assay_AMS.xlsx

Related Article: Tanner C. Jankins, Raul Martin-Montero, Phillippa Cooper, Ruben Martin, Keary M. Engle|2021|J.Am.Chem.Soc.|143|14981|doi:10.1021/jacs.1c07162

Complete list of transcripts with significantly different expression between DTCs and primary tumors, and metastasis and primary tumor by microarray analysis. (XLSX 532 kb)

GPS telemetry markedly enhances the temporal and spatial resolution of animal location data, and recent advances in micro-GPS receivers permit their deployment on small mammals. One such technological advance, snapshot technology, allows for improved battery life by reducing the time to first fix via postponing recovery of satellite ephemeris (satellite location) data and processing of locations. However, no previous work has employed snapshot technology for small, terrestrial mammals. We evaluated performance of two types of micro-GPS (< 20 g) receivers (traditional and snapshot) on a small, semi-fossorial lagomorph, the pygmy rabbit (Brachylagus idahoensis), to understand how GPS errors might influence fine-scale assessments of space use and habitat selection. During stationary tests, microtopography (i.e., burrows) and satellite geometry had the largest influence on GPS fix success rate (FSR) and location error (LE). There was no difference between FSR while animals wore the GPS collars above ground (determined via light sensors) and FSR generated during stationary, above-ground trials, suggesting that animal behavior other than burrowing did not markedly influence micro-GPS errors. In our study, traditional micro-GPS receivers demonstrated similar FSR and LE to snapshot receivers, however, snapshot receivers operated inconsistently due to battery and software failures. In contrast, the initial traditional receivers deployed on animals experienced some breakages, but a modified collar design consistently functioned as expected. If such problems were resolved, snapshot technology could reduce the tradeoff between fix interval and battery life that occurs with traditional micro-GPS receivers. Our results suggest that micro-GPS receivers are capable of addressing questions about space use and resource selection by small mammals, but that additional techniques might be needed to identify use of habitat structures (e.g., burrows, tree cavities, rock crevices) that could affect micro-GPS performance and bias study results. McMahon LA, Rachlow JL, Shipley LA, Forbey JS, Johnson TR, Olsoy PJ (2017) Evaluation of micro-GPS receivers for tracking small-bodied mammals. PLoS ONE. doi:10.1371/journal.pone.0173185