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Data, analysis and figures for pre-print access.

Data for the paper "Rapid hippocampal plasticity supports motor sequence learning"

Bazin fitting routine applied to PLAsTiCC transients.

Data set from the article Moons P, Luyckx K, Thomet C, Budts W, Enomoto J, Sluman MA, Lu CW, Jackson JL, Khairy P, Cook SC, Chidambarathanu S, Alday L, Eriksen K, Dellborg M, Berghammer M, Johansson B, Mackie AS, Menahem S, Caruana M, Veldtman G, Soufi A, Fernandes SM, White K, Callus E, Kutty S, Ombelet F, Apers S, Kovacs AH; APPROACH-IS Consortium and the International Society for Adult Congenital Heart Disease (ISACHD). Physical Functioning, Mental Health, and Quality of Life in Different Congenital Heart Defects: Comparative Analysis in 3538 Patients From 15 Countries. Can J Cardiol. 2021 Feb;37(2):215-223. doi: 10.1016/j.cjca.2020.03.044. Epub 2020 Apr 6. PMID: 32739453. This is the abstract: Background: We compared physical functioning, mental health, and quality of life (QoL) of patients with different subtypes of congenital heart disease (CHD) in a large international sample and investigated the role of functional class in explaining the variance in outcomes across heart defects. Methods: In the cross-sectional Assessment of Patterns of Patient-Reported Outcome in Adults with Congenital Heart Disease-International Study (APPROACH-IS), we enrolled 4028 adult patients with CHD from 15 countries. Diagnostic groups with at least 50 patients were included in these analyses, yielding a sample of 3538 patients (median age: 32 years; 52% women). Physical functioning, mental health, and QoL were measured with the SF-12 health status survey, Hospital Anxiety and Depression Scale (HADS), linear analog scale (LAS) and Satisfaction with Life Scale, respectively. Functional class was assessed using the patient-reported New York Heart Association (NYHA) class. Multivariable general linear mixed models were applied to assess the relationship between the type of CHD and patient-reported outcomes, adjusted for patient characteristics, and with country as random effect. Results: Patients with coarctation of the aorta and those with isolated aortic valve disease reported the best physical functioning, mental health, and QoL. Patients with cyanotic heart disease or Eisenmenger syndrome had worst outcomes. The differences were statistically significant, above and beyond other patient characteristics. However, the explained variances were small (0.6% to 4.1%) and decreased further when functional status was added to the models (0.4% to 0.9%). Conclusions: Some types of CHD predict worse patient-reported outcomes. However, it appears that it is the functional status associated with the heart defect rather than the heart defect itself that shapes the outcomes.

ABSTRACT Both natural and artificial selection are among the main driving forces shaping genetic variation across the genome of livestock species. Selection typically leaves signatures in the genome, which are often characterized by high genetic differentiation across breeds and/or a strong reduction in genetic diversity in regions associated with traits under intense selection pressure. In this study, we evaluated selection signatures and genomic inbreeding coefficients, based on runs of homozygosity (ROH), in six Ugandan goat breeds: Boer (n = 13), and the indigenous breeds Karamojong (n = 15), Kigezi (n = 29), Mubende (n = 29), Small East African (n = 29) and Sebei (n = 29). After genotyping quality control, 45,294 autosomal single nucleotide polymorphisms (SNPs) remained for further analyses. A total of 394 and 6 breed-specific putative selection signatures were identified across all breeds, based on the fixation index ( -values) and hapFLK statistics respectively. These regions were enriched with genes involved in signalling pathways associated directly or indirectly with environmental adaptation, such as immune response (e.g. IL10RB and IL23A), growth and fatty acid composition (e.g. FGF9 and IGF1), and thermo-tolerance (e.g. MTOR and MAPK3). The study revealed little overlap in genomic regions under selection and generally did not display the typical classic selection signatures as expected due to the complex nature of the traits. In the Boer breed, candidate genes associated with production traits, such as body size and growth (e.g. GJB2 and GJA3) were also identified. Furthermore, analysis of ROH in indigenous goat breeds showed very low levels of genomic inbreeding (with the mean per breed ranging from 0.8% to 2.4%), as compared to higher inbreeding in Boer (mean = 13.8%). And, short ROH were more frequent than long ROH, except in Karamojong, providing insight in the developmental history of these goat breeds. This study provides important insights into the effects of long-term selection in Boer and indigenous Ugandan goat breeds and its genetic. Our findings are of great relevance to the implementation of breeding programs and conservation of genetic resources, as well as their sustainable use and management. Funding for the study came from National Agricultural Research Organization (NARO) in Uganda, through a World Bank-supported project, Agricultural Technology and Agribusiness Advisory Services (ATAAS) (P109224).

Cet ensemble de donnée contient les données issues des entrevues que nous avons réalisé avec des spécialistes retrouvés dans le groupe Facebook de machines à écrire.

In order to identify candidate protein binding partners of the transcription factor HEB, we generated conditional HEBAlt-HA transgenic mice. The transgene consisted of an HA-tagged HEBAlt coding region inserted downstream of a ROSA26-loxP-stop-loxP sequence to enable Cre recombinase activated expression of the transgene. Mice with two transgenic alleles without Cre are termed "TT" whereas those bred onto the Vav-Cre background are termed "TTV". Whole thymocytes were obtained from TT (n=2) or TTV (n=2) mice and subjected to immunoprecipitation using an anti-HA antibody and magnetic beads. Samples were trypsin-digested and analyzed by mass spectrometry on a Q Exactive HF-X Mass Spectrometer by the SPARC BioCentre facility (SickKids, Toronto). Scaffold (version Scaffold_4.8.9, Proteome Software Inc., Portland, OR) was used to validate MS/MS based peptide and protein identifications. Protein probabilities were assigned by the Protein Prophet algorithm81.

The images of the bed surface are reported with the following code: eX_hYY, where X is the number of the experimental run and YY is the hour at which the image was taken.

Data set from the article Bernardini A, Camporeale A, Pieroni M, Pieruzzi F, Figliozzi S, Lusardi P, Spada M, Mignani R, Burlina A, Carubbi F, Battaglia Y, Graziani F, Pica S, Tondi L, Chow K, Boveri S, Olivotto I, Lombardi M. Atrial Dysfunction Assessed by Cardiac Magnetic Resonance as an Early Marker of Fabry Cardiomyopathy. JACC Cardiovasc Imaging. 2020 Oct;13(10):2262-2264. doi: 10.1016/j.jcmg.2020.05.011. Epub 2020 Jun 17. PMID: 32563647. Brief introduction Anderson-Fabry disease (AFD) cardiomyopathy is characterized by glycosphingolipid (Gb3) storage in all cellular components, with consequent left ventricular hypertrophy (LVH). Gb3 accumulation also involves atrial myocytes (1), ultimately leading to left atrial (LA) enlargement and reduced atrial compliance. Cardiac magnetic resonance (CMR) plays an important role in the assessment of the severity of Fabry cardiomyopathy. CMR feature tracking (CMR-FT) specifically allows the assessment of myocardial strain from cine images

Datasets for the manuscript Eirew et al Accurate determination of CRISPR-mediated gene fitness in transplantable tumours: - targeted sequencing FASTQ files - sgRNA-UMI count files - sgRNA library content - experiment description