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In vivo PAI of murine bladder

Hall effect data used for Deliverable 3.3 of HITIME project.

Research data for used for the publication: "Grating-graphene metamaterial as a platform for terahertz nonlinear photonics". Datasets measured at the TELBE accelerator-based THz source are measured using a pulse-resolved detection scheme. The data points are sorted according to the absolute arrival time measurement. The four columns hold the following data: 1) absolute time in picoseconds 2) Signal of the emitted THz measured by electro-optic sampling. 3) Relative THz intensity measured using a pyroelectric detector. THz intensity is proportional to absolute power of the signal. 4) Data corresponding to the relative timing of each pulse. Not used for further analysis of the data. The filenumbers of the measurements used for the figures are as follows: Fig. 1a: File 003 Fig. 1b-c: File 043 Fig. 1d-e: File 086 Fig. 2a: Files 080..088 (bare graphene) and 031..049 (grating) Fig. 2b: same Fig. 3a: Files 050..057 Fig. 3c: Files 076..079 Fig. 4. Table top measurements; no file numbers Supp. Fig. 2: File 030 Supp. Fig. 4: File 024..025 Supp. Fig. 7a: Files 031..034 and 036..041 Supp. Fig. 7b: Files 080..083 Supp. Fig. 7d: Files 031..049 and 080..086 Supp. Fig. 9a: Files 031..046 Supp. Fig. 9b: Files 080..085 and 087..088 The datasets measured using a table-top laser source contain two columns. The first one is the position of the optical delay stage in mm that has to be multiplied by 6.667 ps/mm to define the time axis. The second column contains the THz signal as measured using electro-optic sampling.

A) Adhesion of MB49-Luc and 5637 cells to microtiterplates coated with various amounts of GNRs@Chit-Iso4 or GNRs@Chit-Cys. Representative images of cell adhesion to 250 ��g/ml of gold nanorods (according to dry matter content) and the quantification of cell adhesion are shown (Circles, mean��SE, n=3). The EC50 values reported in each plot are the result of 3-4 independent experiments (mean��SE). B) Effect of free Iso4 on MB49-Luc and 5637 cell adhesion to microtiterplates coated with 250 ��g/ml of GNRs@Chit-Iso4 or GNR@Chit-Cys. MB49-Luc and 5637 cells were mixed with the indicated amount of free Iso4 and left to adhere to microtiter plates coated with GNRs. A representative experiment out of three independent experiments is shown (Circles, mean��SE, n=2). C) Binding of various amounts of Iso4-HRP or ARA-HRP to microtiter plates coated with or without a5b1 as detected with OPD chromogenic substrate (Circles, mean��SE, n=2). D) Effect of human urine on the binding of Iso4-HRP to microtiter plates coated with or without a5b1. Iso4-HRP (300 mM) was mixed with the indicated amount of urine, and the mixtures were added to the plates. After washing, bound peroxidase was detected by OPD chromogenic substrate (Bars, mean��SE, n=2). E) Effect of human urine on MB49-Luc cell adhesion to solid-phase coated with GNRs@Chit-Iso4 or GNRs@Chit-Cys. Cells were suspended in DMEM containing 0.1% BSA (upper panel) or in 25 mM Hepes buffer, pH 7.4, containing 150 mM sodium chloride, 1 mM magnesium chloride, 1 mM manganese chloride and 0.1% BSA (lower panel) and spiked with the indicated amounts of human urine. The mixtures were added to microtiter plates coated with 250 ��g/ml of GNRs and left to incubate for 1-2 h at 37��C, 5% CO2. After washing, the adherent cells were fixed and stained with crystal violet. Representative images of cell adhesion to wells coated with 250 ��g/ml of gold nanorods and the quantification of cell adhesion are shown (Bars, mean��SE, n=3). *, P<0.05; **, P<0.01; ***, P<0.001 by two tailed t-test as determined using the GraphPad Prism software.

Biometric features of all the tasks (depending on the subject) computed from the available physiological signals.

This dataset has been created by POLIMI in collaboration with AIDIMME and AIMEN and belongs to Work Package 1 about the definition of a thermal cycle to reduce defect formation LBW and PBF-LB/M processes. This dataset covers data related to process parameters and conditions for melting the materials IN713LC and CM247LC by means of laser powder bed fusion (PBF-LB/M) technology and 304L stainless steel by Laser beam welding (LBW). These include experimental results and the thermal cycles measured within the materials (considering solidification, cooling and following heating cycles induced by adjacent laser scans) with the aim of designing the temporal and spatial energy delivery required from the beam. �� COPYRIGHT 2021 The CUSTODIAN Consortium. All rights reserved.

Hi-C datasets generated in Sungalee et al. are provided as intra-chromosomal contact lists and matrices, divided by cell line/condition and chromosome. The following lymphoma cell lines/conditions are available: - WSU-DLCL2 (WSU_A485 treatment) - Karpas-422 (Karpas422_A485 treatment) Contact lists are provided as compressed plain-text files in which each row represents a Hi-C contact. Column fields are described below: 1) strand of read 1 (0=forward, 16=reverse) 2) chromosome of read 1 3) position on the chromosome where read 1 aligned 4) strand of read 2 (0=forward, 16=reverse) 5) chromosome of read 2 6) position on the chromosome where read 2 aligned 7) mapping quality of read 1 8) name of the read pair as seen in the FASTQ files 9) mapping quality of read 2

This record contains data set related to the article "Single-cell profiling identifies impaired adaptive NK cells expanded after HCMV reactivation in haploidentical HSCT" Haploidentical hematopoietic stem cell transplantation (h-HSCT) represents an efficient curative approach for patients affected by hematologic malignancies in which the reduced intensity conditioning induces a state of immunologic tolerance between donor and recipient. However, opportunistic viral infections greatly affect h-HSCT clinical outcomes. NK cells are the first lymphocytes that recover after transplant and provide a prompt defense against human cytomegalovirus (HCMV) infection/reactivation. By undertaking a longitudinal single-cell computational profiling of multiparametric flow cytometry, we show that HCMV accelerates NK cell immune reconstitution together with the expansion of CD158b1b2jpos/NKG2Aneg/NKG2Cpos/NKp30lo NK cells. The frequency of this subset correlates with HCMV viremia, further increases in recipients experiencing multiple episodes of viral reactivations, and persists for months after the infection. The transcriptional profile of FACS-sorted CD158b1b2jpos NK cells confirmed the ability of HCMV to deregulate NKG2C, NKG2A, and NKp30 gene expression, thus inducing the expansion of NK cells with adaptive traits. These NK cells are characterized by the downmodulation of several gene pathways associated with cell migration, the cell cycle, and effector-functions, as well as by a state of metabolic/cellular exhaustion. This profile reflects the functional impairments of adaptive NK cells to produce IFN-��, a phenomenon also due to the viral-induced expression of lymphocyte-activation gene 3 (LAG-3) and programmed cell death protein 1 (PD-1) checkpoint inhibitors. This data set is composed by .fcs flow cytometry files.

Figure 6C. GRNs deposition in the murine bladder in the unshaken condition.