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Abstract Background Behavioural and structural factors related to sex work, place female sex workers (FSWs) at high risk of maternal mortality and morbidity (MMM), with a large portion due to unintended pregnancies and abortions. In the African context where MMM is the highest in the world, understanding the frequency and determinants of pregnancy and abortion among FSWs is important in order to meet their sexual and reproductive health needs. Methods Data from two Beninese cross-sectional surveys among FSWs aged 18+ (2013, N = 450; 2016, N = 504) were merged. We first performed exploratory univariate analyses to identify factors associated with pregnancy and abortion (p

Additional Figure 4. MRI (top), FDG PET (middle), tau PET (bottom) comparisons between young CN and EOnonAD and old CN and LOnonAD groups. The significance maps show p

Abstract Background PAX6 is a homeodomain transcription factor that acts in a highly dosage-sensitive manner to regulate the development and function of the eyes, nose, central nervous system, gut, and endocrine pancreas. Several individual microRNAs (miRNA) have been implicated in regulating PAX6 in different cellular contexts, but a more general view of how they contribute to the fine-tuning and homeostasis of PAX6 is poorly understood. Results Here, a comprehensive analysis of the Pax6 3′ untranslated region was performed to map potential miRNA recognition elements and served as a backdrop for miRNA expression profiling experiments to identify potential cell/tissue-specific miRNA codes. Pax6 3’UTR pull-down studies identified a cohort of miRNA interactors in pancreatic αTC1–6 cells that, based on the spacing of their recognition sites in the Pax6 3’UTR, revealed 3 clusters where cooperative miRNA regulation may occur. Some of these interacting miRNAs have been implicated in α cell function but have not previously been linked to Pax6 function and may therefore represent novel PAX6 regulators. Conclusions These findings reveal a regulatory landscape upon which miRNAs may participate in the developmental control, fine-tuning and/or homeostasis of PAX6 levels.

Additional file 2: Table S2. Prevalence of classified genera in datasets processed by Qiime1 and Qiime2 after excluding OTUs/ASVs with less than 20 reads across each dataset. Table S4. Prevalence of classified genera in datasets processed by Qiime1 and Qiime2 after excluding OTUs/ASVs with less than 20 reads across each dataset and mean relative abundance of less than 0.01%.

Additional file 3: Figure S3. Vascularization of Notch1[12f/12f] embryos. Embryos were isolated at E10.5, their yolk sacs removed for genotyping, the tip of the PSM was removed for western analysis (see Additional file 4; Figure S4). Staining of fixed embryos with anti-PECAM1 antibody was performed as described previously [1].1. Ge C, Stanley P: Effects of varying Notch1 signal strength on embryogenesis and vasculogenesis in compound mutant heterozygotes. BMC Dev Biol 2010, 10(1):36.