handle: 10062/67716
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handle: 10062/86020
N-atsetüülglükoosamiini või N-galaktoosamiini sisaldavad molekulid on looduses laialt levinud esinedes näiteks kitiinis ning need on olulised nii rakkude füsioloogias kui ka biotehnoloogias. N-atsetüülheksoosaminidaasid (Nah-id) on ensüümid, mis lagundavad glükosiidsidet N atsetüülitud suhkrutes. Bakalaureusetöö põhieesmärk oli kindlaks teha bakteriaalse Nah-i EaNah20 kineetilised parameetrid ja temperatuuristabiilsus ning hinnata valitud punktmutatsioonide mõju ensüümi omadustele. Töös tehti kindlaks, et puhastatud EaNah20 on kõrge katalüütilise aktiivsusega, selle afiinsus sünteetilise substraadi suhtes on 0.35 mM ja ensüüm on stabiilne kuni temperatuurini 50°C. Aspartaadi asendus valgu positsioonis 166 inaktiveeris ensüümi. Proliini asendus positsioonis 248 vähendas ensüümi katalüütilist efektiivsust 25 korda ja alandas temperatuuristabiilsust ligi 6°C võrra.
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handle: 10062/68811
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handle: 10062/73095
Bakalaureusetöös käsitletakse parameetriliste Bezier’ liitkõverate konstrueerimist. Defineeritakse Bernsteini baaspolünoomi, Bezier’ kõvera, polaarkuju ja Bezier’ liitkõvera mõisted ning vaadeldakse nende tähtsamaid omadusi ja nendevahelisi seoseid. Tutvustatakse de Casteljau algoritmi ja uuritakse Bezier’ kõverate ühendamist ja nii ruut- kui ka kuupkõveratega interpoleerimist. Defineeritakse subdivision kui osadeks jaotamine ning ka kui meetod olemasoleva polügoni (murdjoone) parendamiseks tippude lisamise teel. Tutvustatakse nelja subdivision algoritmi.
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handle: 10062/68160
https://www.ester.ee/record=b5359440*est
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handle: 10062/82982
https://www.ester.ee/record=b5508306*est
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handle: 10062/64201
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handle: 10062/64190
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handle: 10062/64910
https://www.ester.ee/record=b5248383*est
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handle: 10062/63856
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handle: 10062/67716
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handle: 10062/86020
N-atsetüülglükoosamiini või N-galaktoosamiini sisaldavad molekulid on looduses laialt levinud esinedes näiteks kitiinis ning need on olulised nii rakkude füsioloogias kui ka biotehnoloogias. N-atsetüülheksoosaminidaasid (Nah-id) on ensüümid, mis lagundavad glükosiidsidet N atsetüülitud suhkrutes. Bakalaureusetöö põhieesmärk oli kindlaks teha bakteriaalse Nah-i EaNah20 kineetilised parameetrid ja temperatuuristabiilsus ning hinnata valitud punktmutatsioonide mõju ensüümi omadustele. Töös tehti kindlaks, et puhastatud EaNah20 on kõrge katalüütilise aktiivsusega, selle afiinsus sünteetilise substraadi suhtes on 0.35 mM ja ensüüm on stabiilne kuni temperatuurini 50°C. Aspartaadi asendus valgu positsioonis 166 inaktiveeris ensüümi. Proliini asendus positsioonis 248 vähendas ensüümi katalüütilist efektiivsust 25 korda ja alandas temperatuuristabiilsust ligi 6°C võrra.
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handle: 10062/68811
https://www.ester.ee/record=b5367447*est
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handle: 10062/73095
Bakalaureusetöös käsitletakse parameetriliste Bezier’ liitkõverate konstrueerimist. Defineeritakse Bernsteini baaspolünoomi, Bezier’ kõvera, polaarkuju ja Bezier’ liitkõvera mõisted ning vaadeldakse nende tähtsamaid omadusi ja nendevahelisi seoseid. Tutvustatakse de Casteljau algoritmi ja uuritakse Bezier’ kõverate ühendamist ja nii ruut- kui ka kuupkõveratega interpoleerimist. Defineeritakse subdivision kui osadeks jaotamine ning ka kui meetod olemasoleva polügoni (murdjoone) parendamiseks tippude lisamise teel. Tutvustatakse nelja subdivision algoritmi.
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influence | Average | |
impulse | Average |
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handle: 10062/68160
https://www.ester.ee/record=b5359440*est
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handle: 10062/82982
https://www.ester.ee/record=b5508306*est
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handle: 10062/64201