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27 Research products

  • 2014-2023
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  • Authors: Kuang, Enjun;
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  • Authors: Mu Rui,; Liu, Shangwu; Jiang Lili,; Wan Shuming,; +6 Authors

    Potato viruses and viroid disease had seriously affected the production of potato in China. In order to cultivate virus and viroids antiviral new materials of potato. In this study, we utilized the strategy of artificial miRNA (amiRNA) with the silencing suppressor P25 protein and HC-pro protein which encoded by potato viral pathogens potato virus X (PVX) and potato virus Y (PVY) as the target, and we utilized 5'RACE technique to forecast the potential shear hot spots of P25 gene and HC-Pro gene. Then we used overlapping PCR technology, and also using Arabidopsis thaliana miR159a precursor as skeleton to design specific primers, so we could construct amiRNA expression vector which was respectively targeting P25 and HC-Pro genes. We constructed the amiRNA expression vector targeting the endogenous gene Virp1 of potato at the same time. And we could obtain amiRNAs three by seriesing three amiRNA expression vector which were construction completed. And then we transferred into the Agrobacterium strain EHA105 respectively, we used Agrobacterium mediated transformation of potato varieties Kexin13. Detected by PCR, the results showed that the target gene was successfully transferred into potato.

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  • Authors: Kuang, Enjun; Chi, Feng-Qin; Zhang, Jiu-Ming; Xu, Ming-Gang; +4 Authors

    Taking black soil as the research object, the difference in the three-dimensional fluorescence spectrum of soil dissolved organic carbon (DOC) after returning maize straw to the field at different soil depths (0~2, 3~10, 11~20, 21~30 and 31~40 cm) were analyzed. The change characteristics of humification degree of maize straw return to deep soil were discussed. The results showed that straw return could increase the content of soil DOC. The characteristics of three-dimensional fluorescence spectra showed that there were two kinds of fluorescence components of soil DOC. CK~T4 treatments were humus like components (Ex/Em=250~275/455 nm) and tryptophan like components (Ex/Em=225~237/340~350 nm), while T5 treatment was humus-like components (250~275/455 nm) and tyrosine like components (Ex/Em =225/304 nm), there were small authigenic components at a depth of 31~40 cm, and the humification coefficient was the highest. The fluorescence intensity of soil DOC component C1 increased with the deepening of straw returning depth, while the C2 component showed a fluctuating state, and the fluorescence intensity increased first and then decreased. Soil DOC was affected by both endogenous and exogenous sources (FI>1.4, 0.6

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  • Authors: JIANG Lili,; MU Rui,; Liu, Shangwu; ZHANG Guizhi,; +1 Authors

    In order to obtain potato plants which could be resistant to multiple viruses ( viroid) simultaneous- ly,P25,HC-Pro,and Virp1 gene targeting PVX,PVY,and PSTVd proteins were cloned,respectively. Three types of amiRNAs targeting sequences encoding the silencing suppressor P25,HC-Pro and Virp1 were designed by using Ar- abidopsis thaliana miR159a as backbone. These three amiRNA sequences were connected by Overlapping PCR. The synthetic of P25,HC-Pro and Virp1 gene was inserted into the expression vector pCAMBIA1300-221 to form p1300-221-preamiR-P25-HCPro-Virp1,and the vector was verified by PCR and restriction enzyme digestion. Pre-a- miR-P25-HCPro-Virp1 was transformed into minituber of potato cultivar Favorita by Agrobacterium tumefaciens in- fection. 15 transformed plants were obtained through regenerating,pressure screening and differentiation. PCR re- sults showed that 10 of them were detected the same size fragment ( 729 bp) as the target gene. Further qRT-PCR t e s t i n g c o n f i r m e d t h a t a m i R - P 2 5 - H C P r o - V i r p 1 g e n e w a s e x p r e s s e d i n 1 0 t r a n s f o r m e d p l a n t s ,a n d t h e r e l a t i v e e x p r e s - sion was between 7. 68 - 21. 37. Inoculated the T0 transgenic plants with the mixture virus of PVX,PVY and PSTVd by friction into the leaves of the plants at the 6 - 8 leaf stage. The plant growth was observed after 20 days and the results showed that the control plants were severely susceptible,with symptoms such as short plants and mottled leaves,while the transformed plants did not show infection symptoms,and growth normally. There was no vi- rus detected by RT-PCR either. This indicated that the transfected amiR-P25-HCPro-Virp could be stably expressed in the transformed plants,and the transformed plants were resistant to PVX,PVY and PVSTd viruses ( viroid) . Transformed plants resistant to PVX,PVY and PVSTd were obtained,and the resistance was significant,which pro- vided new genetic resources for potato virus-resistant breeding.

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  • Authors: Xie, Minmin;

    Tobacco (Nicotiana tabacum L.) is an economic crop in which mature leaves are harvested. Early flowering has serious influence on quality and yield of tobacco. Moreover, long growth cycle is also a major factor limiting the progress of tobacco breeding. Initiation of floral development is regulated through the integration of photoperiod, vernalization, gibberellin and/or autonomous signaling pathways. FT is a key regulatory protein at the intersection of these pathways. Four FT-like genes were isolated from tobacco. Only NtFT4 is a floral inducer and others are floral inhibitors. Tobacco CET, CO, FPF1, LFY, NFL, MADS box and other genes also play very important roles in tobacco flowering. In this article, we summarize the research progress on characteristics, function and regulatory mechanisms of tobacco FT gene. It provided useful information for preventing early flowering and accelerating breeding progress in tobacco.

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  • Authors: Zhang, Peipei;
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  • Authors: Rong, Wei;

    Plant-pathogen interactions have been performed extensively over the years from both plant and pathogen viewpoint. An overall understanding of how plant and pathogen recognize each other, and the differentiate to establish either a compatible or an incompatible relationship is crucial in this field of inverstigation. With transcriptomics approaches, we could successfully identify the defence-related genes expressed during phytopathogen infections. The plant-paghogen interactions and findings considering the wheat sharp eyespot, Rhizoctonia cerealis and transcriptomics were summeriszed in this paper. We also discussed these findings considering the advantages and limitations of current transcriptomic tool in plant-pathogen interactions.

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  • Authors: Braham, Marc; Pierard, Sébastien;

    he present invention concerns a method for assigning a pixel to one of a foreground pixel set and a background pixel set. In this method, if a first condition is met the pixel is assigned to the background pixel set , and if the first condition is not met and a second condition is met, the pixel is assigned to the foreground pixel set. The method comprises a step ( S100 ) of calculating a probability that the pixel belongs to a foreground-relevant object according to a semantic segmentation algorithm, the first condition is that this probability that the pixel belongs to a foreground relevant object does not exceed a first predetermined threshold, and the second condition is that a difference between this probability that the pixel belongs to a foreground relevant object and a baseline probability for the pixel equals or exceeds a second predetermined threshold.

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  • Authors: Xu, Qingxuan; Chevalier Mendes Lopes, Thomas; Hatt, Séverin; Francis, Frédéric; +1 Authors

    Abstract: The concept of pest control has been changed from traditional “kill” to scientific “regulation”. Ecological regulation has become one of the hotspots in the field of sustainable agriculture. Intercropping (i.e., the simultaneous growing of two or more species in the same field for a significant period but without necessarily being sown and harvested at the same time), is one way to increase the plant diversity. In addition to intercropping, semiochemicals (i.e., informative molecules used in insect-insect or plant-insect interactions) have been widely considered within various Integrated Pest Management (IPM) strategies. Laboratory and field studies have demonstrated that releasing semiochemicals has the potential to simultaneously repel pests and attract natural enemies. For instance, methyl salicylate (MeSA) is a herbivore-induced plant volatile that is repellent to Rhopalosiphum padi (L.) and other cereal aphids, while being attractive to aphid predators such as ladybeetles, lacewings and hoverflies, and increasing parasitism rate. Moreover, the aphid alarm pheromone, which major component is E-β-farnesene (EBF), acts as a repellent for plant herbivores, and attracts predatory beetles, hoverflies and increase parasitism rate. Combining both strategies could be an interesting way to maximize pest control. In a 2 year set-up (March-August 2015, 2016), a wheat-pea strip intercropping combined with the release of EBF or MeSA was tested in Gembloux to improve push-pull strategy by repelling aphids and by attracting beneficials simultaneously. The results showed that the abundance of aphids, lacewings and mummified aphids on both pea plants and wheat tillers were all affected by the release of oil formulated EBF and MeSA. Parasitism rate was also affected by the treatments on both crops in both years. The occurrence of wheat aphids and pea aphids were reduced by 35% and 30%. In the case of wheat and pea, pea aphids peak earlier than wheat ones (in the present study on plants, pea aphids: 17 June 2015 and 3 June 2016, while wheat aphids: 25 June 2015 and 10 June 2016), hence associating these two crops allows natural enemies to quickly move from pea to wheat and controlling pests in the course of their occurrence, in fine protecting both crops. Wheat–pea intercropping combined with EBF oil and MeSA release, enhanced the abundance of predators and parasitoids on the pea, our results strongly support the efficacy of combining intercropping and semiochemical release to attain improved pest control. Key words: Ecological regulation; Methyl salicylate; Aphid alarm pheromone; Push-pull; Green control 中比国际科技合作项目(2014DFG32270)

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  • Authors: Guo, Jianqing; Wang, Zhenying;
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27 Research products
  • Authors: Kuang, Enjun;
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  • Authors: Mu Rui,; Liu, Shangwu; Jiang Lili,; Wan Shuming,; +6 Authors

    Potato viruses and viroid disease had seriously affected the production of potato in China. In order to cultivate virus and viroids antiviral new materials of potato. In this study, we utilized the strategy of artificial miRNA (amiRNA) with the silencing suppressor P25 protein and HC-pro protein which encoded by potato viral pathogens potato virus X (PVX) and potato virus Y (PVY) as the target, and we utilized 5'RACE technique to forecast the potential shear hot spots of P25 gene and HC-Pro gene. Then we used overlapping PCR technology, and also using Arabidopsis thaliana miR159a precursor as skeleton to design specific primers, so we could construct amiRNA expression vector which was respectively targeting P25 and HC-Pro genes. We constructed the amiRNA expression vector targeting the endogenous gene Virp1 of potato at the same time. And we could obtain amiRNAs three by seriesing three amiRNA expression vector which were construction completed. And then we transferred into the Agrobacterium strain EHA105 respectively, we used Agrobacterium mediated transformation of potato varieties Kexin13. Detected by PCR, the results showed that the target gene was successfully transferred into potato.

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  • Authors: Kuang, Enjun; Chi, Feng-Qin; Zhang, Jiu-Ming; Xu, Ming-Gang; +4 Authors

    Taking black soil as the research object, the difference in the three-dimensional fluorescence spectrum of soil dissolved organic carbon (DOC) after returning maize straw to the field at different soil depths (0~2, 3~10, 11~20, 21~30 and 31~40 cm) were analyzed. The change characteristics of humification degree of maize straw return to deep soil were discussed. The results showed that straw return could increase the content of soil DOC. The characteristics of three-dimensional fluorescence spectra showed that there were two kinds of fluorescence components of soil DOC. CK~T4 treatments were humus like components (Ex/Em=250~275/455 nm) and tryptophan like components (Ex/Em=225~237/340~350 nm), while T5 treatment was humus-like components (250~275/455 nm) and tyrosine like components (Ex/Em =225/304 nm), there were small authigenic components at a depth of 31~40 cm, and the humification coefficient was the highest. The fluorescence intensity of soil DOC component C1 increased with the deepening of straw returning depth, while the C2 component showed a fluctuating state, and the fluorescence intensity increased first and then decreased. Soil DOC was affected by both endogenous and exogenous sources (FI>1.4, 0.6

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  • Authors: JIANG Lili,; MU Rui,; Liu, Shangwu; ZHANG Guizhi,; +1 Authors

    In order to obtain potato plants which could be resistant to multiple viruses ( viroid) simultaneous- ly,P25,HC-Pro,and Virp1 gene targeting PVX,PVY,and PSTVd proteins were cloned,respectively. Three types of amiRNAs targeting sequences encoding the silencing suppressor P25,HC-Pro and Virp1 were designed by using Ar- abidopsis thaliana miR159a as backbone. These three amiRNA sequences were connected by Overlapping PCR. The synthetic of P25,HC-Pro and Virp1 gene was inserted into the expression vector pCAMBIA1300-221 to form p1300-221-preamiR-P25-HCPro-Virp1,and the vector was verified by PCR and restriction enzyme digestion. Pre-a- miR-P25-HCPro-Virp1 was transformed into minituber of potato cultivar Favorita by Agrobacterium tumefaciens in- fection. 15 transformed plants were obtained through regenerating,pressure screening and differentiation. PCR re- sults showed that 10 of them were detected the same size fragment ( 729 bp) as the target gene. Further qRT-PCR t e s t i n g c o n f i r m e d t h a t a m i R - P 2 5 - H C P r o - V i r p 1 g e n e w a s e x p r e s s e d i n 1 0 t r a n s f o r m e d p l a n t s ,a n d t h e r e l a t i v e e x p r e s - sion was between 7. 68 - 21. 37. Inoculated the T0 transgenic plants with the mixture virus of PVX,PVY and PSTVd by friction into the leaves of the plants at the 6 - 8 leaf stage. The plant growth was observed after 20 days and the results showed that the control plants were severely susceptible,with symptoms such as short plants and mottled leaves,while the transformed plants did not show infection symptoms,and growth normally. There was no vi- rus detected by RT-PCR either. This indicated that the transfected amiR-P25-HCPro-Virp could be stably expressed in the transformed plants,and the transformed plants were resistant to PVX,PVY and PVSTd viruses ( viroid) . Transformed plants resistant to PVX,PVY and PVSTd were obtained,and the resistance was significant,which pro- vided new genetic resources for potato virus-resistant breeding.

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  • Authors: Xie, Minmin;

    Tobacco (Nicotiana tabacum L.) is an economic crop in which mature leaves are harvested. Early flowering has serious influence on quality and yield of tobacco. Moreover, long growth cycle is also a major factor limiting the progress of tobacco breeding. Initiation of floral development is regulated through the integration of photoperiod, vernalization, gibberellin and/or autonomous signaling pathways. FT is a key regulatory protein at the intersection of these pathways. Four FT-like genes were isolated from tobacco. Only NtFT4 is a floral inducer and others are floral inhibitors. Tobacco CET, CO, FPF1, LFY, NFL, MADS box and other genes also play very important roles in tobacco flowering. In this article, we summarize the research progress on characteristics, function and regulatory mechanisms of tobacco FT gene. It provided useful information for preventing early flowering and accelerating breeding progress in tobacco.

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  • Authors: Zhang, Peipei;
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  • Authors: Rong, Wei;

    Plant-pathogen interactions have been performed extensively over the years from both plant and pathogen viewpoint. An overall understanding of how plant and pathogen recognize each other, and the differentiate to establish either a compatible or an incompatible relationship is crucial in this field of inverstigation. With transcriptomics approaches, we could successfully identify the defence-related genes expressed during phytopathogen infections. The plant-paghogen interactions and findings considering the wheat sharp eyespot, Rhizoctonia cerealis and transcriptomics were summeriszed in this paper. We also discussed these findings considering the advantages and limitations of current transcriptomic tool in plant-pathogen interactions.

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