Lists of woody plants produced in nurseries were preserved from the years 1794, 1800 and 1814 in the manor of Nové Dvory of the noble family of Chotek. 276 taxa of woody plants in the current concept, permanently cultivated outdoors, have been determined in these lists at least to the level of the species. According to the existing findings, for 241 of them were documented for the first time their production for the needs of the landscape architecture in the territory of the Czech Republic. In the case of foreign natural and all cultural taxa, it is also the oldest evidence of their presence in this territory; for native taxa it is the first evidence of their usage in garden culture. Approximately 21.5% of taxa are autochthonous in the Czech Republic, 24% have at least part of their native territory in Europe and 1.5% in the Middle East, 2% come from Central Asia and Siberia and 4% from East Asia. Taxa produced in culture account for approximately 13%. Woody plants of North American origin (they are given a separate contribution) are represented by 34%.