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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Denisa Šimoníková; Alžbeěta Němečková; Miroslava Karafiátová; Brigitte Uwimana; +5 Authors

    Oligo Painting FISH a été établi pour identifier tous les chromosomes de la banane (Musa spp.) et pour ancrer les pseudomolécules de la séquence du génome de référence de Musa acuminata spp. malaccensis « DH Pahang » aux chromosomes individuels in situ. Un total de 19 sondes de peinture oligo spécifiques aux chromosomes/bras chromosomiques ont été développées et se sont révélées appropriées pour les études de cytogénétique moléculaire dans le genre Musa. Pour la première fois, des caryotypes moléculaires de M. acuminata spp. malaccensis diploïde (génome A), M. balbisiana (génome B) et M. schizocarpa (génome S) de la section Eumusa de Musa, qui ont contribué à l'évolution de cultivars de bananes comestibles, ont été établis. Ceci a été réalisé après une utilisation combinée de sondes de peinture oligo et d'un ensemble de marqueurs cytogénétiques de la banane précédemment développés. La densité des sondes de peinture oligo était suffisante pour étudier les réarrangements chromosomiques sur les chromosomes mitotiques ainsi que sur les chromosomes pachytènes méiotiques. Cette avancée permettra la cartographie comparative des POISSONS et l'identification des translocations chromosomiques qui ont accompagné l'évolution du génome et la spéciation dans la famille des Musaceae. Oligo Paint FISH se estableció para identificar todos los cromosomas en el plátano (Musa spp.) y para anclar las pseudomoléculas de la secuencia del genoma de referencia de Musa acuminata spp. malaccensis "DH Pahang" a los cromosomas individuales in situ. Se desarrollaron un total de 19 sondas de pintura oligoespecíficas de cromosoma/brazo cromosómico y se demostró que eran adecuadas para estudios citogenéticos moleculares en el género Musa. Por primera vez, se establecieron cariotipos moleculares de M. acuminata spp. malaccensis diploide (genoma A), M. balbisiana (genoma B) y M. schizocarpa (genoma S) de la sección Eumusa de Musa, que contribuyeron a la evolución de los cultivares de banano comestible. Esto se logró después de un uso combinado de sondas de pintura oligo y un conjunto de marcadores citogenéticos de plátano desarrollados previamente. La densidad de las sondas de pintura oligo fue suficiente para estudiar los reordenamientos cromosómicos en los cromosomas mitóticos y meióticos del paquiteno. Este avance permitirá el mapeo comparativo de FISH y la identificación de las translocaciones cromosómicas que acompañaron la evolución y especiación del genoma en la familia Musaceae. Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis "DH Pahang" to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae. تم إنشاء أوليجو لرسم الأسماك لتحديد جميع الكروموسومات في الموز (أنواع موسى) وترسيخ الجزيئات الزائفة من تسلسل الجينوم المرجعي من أنواع موسى الأكومينات "دي إتش باهانج" إلى الكروموسومات الفردية في الموقع. تم تطوير ما مجموعه 19 مجس رسم أوليجو خاص بالكروموسوم/ذراع الكروموسوم وتم إثبات أنها مناسبة للدراسات الوراثية الخلوية الجزيئية في جنس موسى. ولأول مرة، تم إنشاء أنماط نواة جزيئية لـ M. Acuminata spp. malaccensis (جينوم A) و M. Balbisiana (جينوم B) و M. schizocarpa (جينوم S) من قسم Eumusa في موسى، مما ساهم في تطور أصناف الموز الصالحة للأكل. وقد تحقق ذلك بعد الاستخدام المشترك لمسابير رسم القلة ومجموعة من العلامات الوراثية الخلوية للموز التي تم تطويرها مسبقًا. كانت كثافة مجسات طلاء القلة كافية لدراسة إعادة ترتيب الكروموسومات على الكروموسومات الانقسامية وكذلك على كروموسومات الباكيتين الانقسامية. سيمكن هذا التقدم من رسم خرائط الأسماك المقارنة وتحديد نقل الكروموسومات التي رافقت تطور الجينوم والانتواع في عائلة Musaceae.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Liriasarrow_drop_down
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    Frontiers in Plant Science
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    Frontiers in Plant Science
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      Frontiers in Plant Science
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      https://doi.org/10.60692/sq653...
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Denisa Šimoníková; Alžbeěta Němečková; Miroslava Karafiátová; Brigitte Uwimana; +5 Authors

    Oligo Painting FISH a été établi pour identifier tous les chromosomes de la banane (Musa spp.) et pour ancrer les pseudomolécules de la séquence du génome de référence de Musa acuminata spp. malaccensis « DH Pahang » aux chromosomes individuels in situ. Un total de 19 sondes de peinture oligo spécifiques aux chromosomes/bras chromosomiques ont été développées et se sont révélées appropriées pour les études de cytogénétique moléculaire dans le genre Musa. Pour la première fois, des caryotypes moléculaires de M. acuminata spp. malaccensis diploïde (génome A), M. balbisiana (génome B) et M. schizocarpa (génome S) de la section Eumusa de Musa, qui ont contribué à l'évolution de cultivars de bananes comestibles, ont été établis. Ceci a été réalisé après une utilisation combinée de sondes de peinture oligo et d'un ensemble de marqueurs cytogénétiques de la banane précédemment développés. La densité des sondes de peinture oligo était suffisante pour étudier les réarrangements chromosomiques sur les chromosomes mitotiques ainsi que sur les chromosomes pachytènes méiotiques. Cette avancée permettra la cartographie comparative des POISSONS et l'identification des translocations chromosomiques qui ont accompagné l'évolution du génome et la spéciation dans la famille des Musaceae. Oligo Paint FISH se estableció para identificar todos los cromosomas en el plátano (Musa spp.) y para anclar las pseudomoléculas de la secuencia del genoma de referencia de Musa acuminata spp. malaccensis "DH Pahang" a los cromosomas individuales in situ. Se desarrollaron un total de 19 sondas de pintura oligoespecíficas de cromosoma/brazo cromosómico y se demostró que eran adecuadas para estudios citogenéticos moleculares en el género Musa. Por primera vez, se establecieron cariotipos moleculares de M. acuminata spp. malaccensis diploide (genoma A), M. balbisiana (genoma B) y M. schizocarpa (genoma S) de la sección Eumusa de Musa, que contribuyeron a la evolución de los cultivares de banano comestible. Esto se logró después de un uso combinado de sondas de pintura oligo y un conjunto de marcadores citogenéticos de plátano desarrollados previamente. La densidad de las sondas de pintura oligo fue suficiente para estudiar los reordenamientos cromosómicos en los cromosomas mitóticos y meióticos del paquiteno. Este avance permitirá el mapeo comparativo de FISH y la identificación de las translocaciones cromosómicas que acompañaron la evolución y especiación del genoma en la familia Musaceae. Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis "DH Pahang" to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae. تم إنشاء أوليجو لرسم الأسماك لتحديد جميع الكروموسومات في الموز (أنواع موسى) وترسيخ الجزيئات الزائفة من تسلسل الجينوم المرجعي من أنواع موسى الأكومينات "دي إتش باهانج" إلى الكروموسومات الفردية في الموقع. تم تطوير ما مجموعه 19 مجس رسم أوليجو خاص بالكروموسوم/ذراع الكروموسوم وتم إثبات أنها مناسبة للدراسات الوراثية الخلوية الجزيئية في جنس موسى. ولأول مرة، تم إنشاء أنماط نواة جزيئية لـ M. Acuminata spp. malaccensis (جينوم A) و M. Balbisiana (جينوم B) و M. schizocarpa (جينوم S) من قسم Eumusa في موسى، مما ساهم في تطور أصناف الموز الصالحة للأكل. وقد تحقق ذلك بعد الاستخدام المشترك لمسابير رسم القلة ومجموعة من العلامات الوراثية الخلوية للموز التي تم تطويرها مسبقًا. كانت كثافة مجسات طلاء القلة كافية لدراسة إعادة ترتيب الكروموسومات على الكروموسومات الانقسامية وكذلك على كروموسومات الباكيتين الانقسامية. سيمكن هذا التقدم من رسم خرائط الأسماك المقارنة وتحديد نقل الكروموسومات التي رافقت تطور الجينوم والانتواع في عائلة Musaceae.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Liriasarrow_drop_down
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    Frontiers in Plant Science
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    Frontiers in Plant Science
    Article . 2019 . Peer-reviewed
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    https://doi.org/10.60692/sq653...
    Other literature type . 2019
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      Frontiers in Plant Science
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      License: CC BY
      Data sources: UnpayWall
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      DOAJ
      Article . 2019
      Data sources: DOAJ
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      Frontiers in Plant Science
      Article . 2019 . Peer-reviewed
      License: CC BY
      Data sources: Crossref
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      DOAJ-Articles
      Article . 2019
      Data sources: DOAJ-Articles
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      https://doi.org/10.60692/sq653...
      Other literature type . 2019
      Data sources: Datacite
      https://doi.org/10.60692/0pxyx...
      Other literature type . 2019
      Data sources: Datacite
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