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Bioluminescence determination of active caspase-3 in single apoptotic cells
descriptionPublicationkeyboard_double_arrow_right Article 2013 Czech Republic English
Authors: Lišková, M. (Marcela); Klepárník, K. (Karel); Matalová, E. (Eva); Hegrová, J. (Jitka); +3 Authors
Lišková, M. (Marcela); Klepárník, K. (Karel); Matalová, E. (Eva); Hegrová, J. (Jitka); Přikryl, J. (Jan); Švandová, E. (Eva); Foret, F. (František);
handle: 11104/0218423
handle: 11104/0218423
In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. The technology is based on the specific cleavage of modified luciferin by caspase-3, an immediate bioluminescence reaction of free luciferin with luciferase followed by emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
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Article . 2013
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Article . 2013
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Bioluminescence determination of active caspase-3 in single apoptotic cells
descriptionPublicationkeyboard_double_arrow_right Article 2013 Czech Republic English
Authors: Lišková, M. (Marcela); Klepárník, K. (Karel); Matalová, E. (Eva); Hegrová, J. (Jitka); +3 Authors
Lišková, M. (Marcela); Klepárník, K. (Karel); Matalová, E. (Eva); Hegrová, J. (Jitka); Přikryl, J. (Jan); Švandová, E. (Eva); Foret, F. (František);
handle: 11104/0218423
handle: 11104/0218423
In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. The technology is based on the specific cleavage of modified luciferin by caspase-3, an immediate bioluminescence reaction of free luciferin with luciferase followed by emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Repository of the Cz...arrow_drop_down
Repository of the Czech Academy of Sciences
Article . 2013
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Repository of the Cz...arrow_drop_down
Repository of the Czech Academy of Sciences
Article . 2013
Data sources: Repository of the Czech Academy of Sciences
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citeCite
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Please grant OpenAIRE to access and update your ORCID works.
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Bioluminescence determination of active caspase-3 in single apoptotic cells

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Bioluminescence determination of active caspase-3 in single apoptotic cells

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Bioluminescence determination of active caspase-3 in single apoptotic cells