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  • 2019-2023
  • SK
  • Corona Virus Disease

  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: N. Ahmad Aziz; Victor M. Corman; Antje K.C. Echterhoff; Anja Richter; +6 Authors

    ABSTRACTBackgroundAccurate estimates of SARS-CoV-2 seroprevalence are crucial for the implementation of effective public health measures, but are currently largely lacking in regions with low infection rates. This is further complicated by inadequate test performance of many widely used serological assays. We therefore aimed to assess SARS-CoV-2 seroprevalence in a region with low COVID-19 burden, especially focusing on neutralizing antibodies that presumably constitute a major component of acquired immunity.MethodsWe invited all individuals who were enrolled in the Rhineland Study, an ongoing community-based prospective cohort study in people aged 30 years and above in the city of Bonn, Germany (N=5427). Between April 24th and June 30th, 2020, 4771 (88%) of these individuals participated in the serosurvey. Anti-SARS-CoV-2 IgG levels were measured using an ELISA assay, and all positive or borderline results were subsequently examined through both a recombinant immunofluorescent assay and a plaque reduction neutralisation test (PRNT).FindingsSeroprevalence was 0·97% (95% CI: 0·72–1·30) by ELISA and 0·36% (95% CI: 0·21–0·61) by PRNT, and did not vary with either age or sex. All PRNT+ individuals reported having experienced at least one symptom (odds ratio (OR) of PRNT+ for each additional symptom: 1·12 (95% CI: 1·04–1·21)). Apart from living in a household with a SARS-CoV-2 confirmed or suspected person, a recent history of reduced taste or smell, fever, chills/hot flashes, pain while breathing, pain in arms/legs, as well as muscle pain and weakness were significantly associated with the presence of neutralizing antibodies in those with mild to moderate infection (ORs 3·44 to 9·97, all p<0·018).InterpretationOur findings indicate a relatively low SARS-CoV-2 seroprevalence in Bonn, Germany (until June 30th, 2020), with neutralizing antibodies detectable in only one third of those with a positive immunoassay result, implying that almost the entire population in this region remains susceptible to SARS-CoV-2 infection.FundingThe Rhineland Study is predominantly funded through the German Center for Neurodegenerative Diseases (DZNE) by the Federal Ministry of Education and Research (BMBF) and the Ministry of Culture and Science of the German State of North Rhine-Westphalia. The National Consultant Laboratory for Coronaviruses is funded by the Federal Ministry of Health (BMG). No additional funding was received for this seroprevalence study.

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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ medRxivarrow_drop_down
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    Authors: Marek Petráš; Ivana Králová Lesná;

    ABSTRACT Immunological memory is the ability of the adaptive immune system to ensure a persistent protective effect after immunization. However, it can also be a limitation to building a sufficient level of protective antibodies specific to new mutations of the virus. It is imperative to bear this phenomenon (called “original antigenic sin”) in mind and make every effort to overcome its inherent pitfalls when updating current and designing new vaccines.

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    Human Vaccines & Immunotherapeutics
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    Human Vaccines & Immunotherapeutics
    Article . 2021 . Peer-reviewed
    License: CC BY NC ND
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      Human Vaccines & Immunotherapeutics
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      Human Vaccines & Immunotherapeutics
      Article . 2021 . Peer-reviewed
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    Authors: Francisco J. Zapatero-Belinchón; Erik Dietzel; Olga Dolnik; Katinka Döhner; +14 Authors

    Filoviruses infect a wide range of cell types with the exception of lymphocytes. The intracellular proteins cathepsin B and L, two-pore channel 1 and 2, and bona fide receptor Niemann⁻Pick Disease C1 (NPC1) are essential for the endosomal phase of cell entry. However, earlier steps of filoviral infection remain poorly characterized. Numerous plasma membrane proteins have been implicated in attachment but it is still unclear which ones are sufficient for productive entry. To define a minimal set of host factors required for filoviral glycoprotein-driven cell entry, we screened twelve cell lines and identified the nonlymphocytic cell line SH-SY5Y to be specifically resistant to filovirus infection. Heterokaryons of SH-SY5Y cells fused to susceptible cells were susceptible to filoviruses, indicating that SH-SY5Y cells do not express a restriction factor but lack an enabling factor critical for filovirus entry. However, all tested cell lines expressed functional intracellular factors. Global gene expression profiling of known cell surface entry factors and protein expression levels of analyzed attachment factors did not reveal any correlation between susceptibility and expression of a specific host factor. Using binding assays with recombinant filovirus glycoprotein, we identified cell attachment as the step impaired in filovirus entry in SH-SY5Y cells. Individual overexpression of attachment factors T-cell immunoglobulin and mucin domain 1 (TIM-1), Axl, Mer, or dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) rendered SH-SY5Y cells susceptible to filovirus glycoprotein-driven transduction. Our study reveals that a lack of attachment factors limits filovirus entry and provides direct experimental support for a model of filoviral cell attachment where host factor usage at the cell surface is highly promiscuous. CA extern

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Europe PubMed Centra...arrow_drop_down
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    Viruses
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    Viruses
    Article . 2019
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    Article . 2019
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      Viruses
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      Viruses
      Article . 2019
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    Authors: Samuel Genzor; Jan Prasko; Jan Mizera; Petr Jakubec; +4 Authors

    Samuel Genzor,1,&ast; Jan Prasko,2– 4,&ast; Jan Mizera,1,&ast; Petr Jakubec,1,&ast; Milan Sova,1,5,&ast; Jakub Vanek,2,&ast; Nikoleta Šurinová,6,&ast; Katerina Langova7,&ast; 1Department of Respiratory Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Olomouc Czech Republic; 2Department of Psychiatry, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc Czech Republic; 3Institute for Postgraduate Education in Health Care, Prague Czech Republic; 4Department of Psychology Sciences, Faculty of Social Science and Health Care, Constantine the Philosopher University in Nitra, Nitra The Slovak Republic; 5Department of Respiratory Medicine, Faculty of Medicine and Dentistry, Masaryk University Brno and University Hospital Brno, Brno Czech Republic; 6Palacky University Olomouc, Olomouc Czech Republic; 7Department of Biophysics, Palacky University Olomouc, Olomouc Czech Republic&ast;These authors contributed equally to this workCorrespondence: Jan Prasko, Department of Psychiatry, Faculty of Medicine and Dentistry, University Hospital Olomouc, I. P. Pavlova 6, Olomouc, 775 20, Czech Republic, Email praskojan@seznam.czBackground: Patients with obstructive sleep apnoea (OSA) are at increased risk of severe course of COVID-19. Vaccination remains to be the most effective prevention of complicated courses of infection. The best contemporary conservative treatment of OSA is continuous positive airway pressure (CPAP) therapy.Purpose: To compare vaccination acceptance and outcomes of COVID-19 infection between OSA patients adhering to the CPAP therapy and those who rejected CPAP and surgical therapy.Patients and Methods: Subjects were divided into two groups: group A (N = 167) were individuals with sufficient CPAP adherence (more than 4 hours per night on average) over the last 10 years. Group B (N = 106) were individuals who did not use the CPAP therapy at all and had no indications to surgical therapy.Results: Three patients in group B died, and one had a severe course of COVID-19. None of the patients in group A died or experienced a severe course of COVID-19. Group A had a significantly higher proportion of males (77.8% compared to 66% in group B) and all parameters of OSA severity. The vaccination status was similar among both groups, with a complete triple dose vaccination rate of 69.5% and 67.9% in groups A and B, respectively.Conclusion: The results show that the patients with OSA adherent to CPAP therapy were less likely to experience a severe course of COVID-19 or death than the OSA patients non-compliant with therapy, despite the former group having more severe OSA. This result underlines the importance of adherence to CPAP therapy in OSA.Keywords: OSA, COVID-19, vaccination, treatment with CPAP, CPAP adherence

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    Patient Preference and Adherence
    Article . 2022 . Peer-reviewed
    Data sources: Dove Medical Press
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    Patient Preference and Adherence
    Article . 2022 . Peer-reviewed
    License: CC BY NC
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    https://doi.org/10.1183/231205...
    Conference object . 2023 . Peer-reviewed
    Data sources: Crossref
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      Patient Preference and Adherence
      Article . 2022 . Peer-reviewed
      Data sources: Dove Medical Press
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      Patient Preference and Adherence
      Article . 2022 . Peer-reviewed
      License: CC BY NC
      Data sources: Crossref
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      https://doi.org/10.1183/231205...
      Conference object . 2023 . Peer-reviewed
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    Authors: Carolin Otto; Tatjana Schwarz; Lara M. Jeworowski; Marie L. Schmidt; +9 Authors

    AbstractBackgroundHumoral immune responses to COVID-19 vaccination are diminished in anti-CD20 treated patients with multiple sclerosis (pwMS). In healthy individuals, neutralizing antibodies against the SARS-CoV-2 Omicron variant are only detected after three COVID-19 vaccinations. It was hitherto unknown whether a third or fourth COVID-19 vaccination of anti-CD20 treated pwMS improves SARS-CoV-2 specific humoral immune responses, including neutralizing antibodies against Omicron.MethodsAnti-CD20 treated pwMS vaccinated two (n=61), three (n=57) or four (n=15) times and healthy controls (n=10) vaccinated thrice were included in a prospective cohort study. Anti-SARS-CoV-2 spike S1 IgG and IgA levels, maturation of SARS-CoV-2 IgG avidity, neutralizing capacity against the SARS-CoV-2 Omicron BA.2 variant and SARS-CoV-2 specific T cell responses were analyzed.ResultsThe proportion of anti-CD20 treated pwMS with detectable SARS-CoV-2 S1 IgG was similar after the second (31/61, 50.8%), third (31/57, 54.4%) and fourth (8/15, 53.3%) vaccination. In pwMS with detectable SARS-CoV-2 IgG, the proportion with high affinity antibodies increased from the second (6/31, 19.4%) to the third (17/31, 54.8%) and fourth (6/8, 75%) vaccination. While none (0/10) of the anti-CD20 treated pwMS vaccinated twice had Omicron specific neutralizing antibodies, 3/10 (30%) pwMS vaccinated thrice and 3/5 (60%) pwMS vaccinated four times generated Omicron specific neutralizing antibodies.ConclusionAlthough SARS-CoV-2 specific humoral immune responses remain quantitatively impaired, in those anti-CD20 treated pwMS who do develop SARS-CoV-2 antibodies, the functionality of SARS-CoV-2 antibodies, including neutralizing antibodies against Omicron, improves after three and four SARS-CoV-2 vaccinations, supporting current recommendations for one or two booster vaccination in anti-CD20 treated pwMS.

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    Multiple Sclerosis Journal
    Article . 2023 . Peer-reviewed
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      Multiple Sclerosis Journal
      Article . 2023 . Peer-reviewed
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    Authors: Robert Flisiak; Dorota Zarębska-Michaluk; Sona Frankova; Ivica Grgurević; +8 Authors

    AbstractAccording to the recent data presented by Central‐European HCV experts, the estimated prevalence of HCV is between 0.2% and 1.7% in certain countries in this region. There are no financial limitations to access to treatment in most countries. Patients in these countries have access to at least one pangenotypic regimen. The most common barriers to the elimination of HCV in Central Europe are a lack of established national screening programmes and limited political commitment to the elimination of HCV. Covid‐19 has significantly affected the number of patients who have been diagnosed and treated, thus, delaying the potential elimination of HCV. These data suggest that the elimination of HCV elimination projected by WHO before 2030 will not be possible in the Central Europe.

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    Liver International
    Article . 2021 . Peer-reviewed
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      Liver International
      Article . 2021 . Peer-reviewed
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    Authors: I Wayan Aditya Tariana;

    The beginning of 2020 was an extraordinary event for the whole world. This is because almost all parts of the world are hit by the Covid-19 outbreak which causes unrest and has a big impact on every field. The COVID-19 pandemic makes people have to maintain their health by washing their hands, maintaining distance, not crowding, and maintaining cleanliness (Abidin, et al., 2021). Buhungo (2012) explained that environmental cleanliness is a condition where the environment is free from various kinds of dirt and disease that can cause losses to the community, both from activities and the environment. The condition of the COVID-19 pandemic forces every community to maintain cleanliness, both their personal hygiene and also the cleanliness of their environment. To provide assistance to the community, especially in the village of Kedisan Kintamani, the Faculty of Economics and BEM-FEB Universitas Pendidikan Nasional (UNDIKNAS) Denpasar collaborate in holding community social activities which are also under the auangan of LP2M and the Dean of FEB UNDIKNAS. The community social activities were held with the theme "Economy Social Care". This activity aims to help the community around Kedisan Kintamani village in maintaining the cleanliness of their surrounding environment. Kondisi Pandemi covid-19 memaksa setiap kalangan masyarakat untuk tetap menjaga kebersihan, baik kebersihan dirinya dan juga kebersihan lingkungan mereka. Untuk memberikan bantuan pada masyarakat terutama pada desa Kedisan Kintamani, Fakultas Ekonomi dan BEM-FEB Universitas Pendidikan Nasional (UNDIKNAS) Denpasar melakukan kolaborasi dalam mengadakan kegiatan sosial masyarakat yang juga berada dibawah nauangan LP2M berserta Dekan FEB UNDIKNAS. Adapun kegiatan sosial masyarakat tersebut diadakan dengan mengusung tema “Economy Social Care”. Kegiatan ini memiliki tujuan untuk membantu masyarakat sekitar desa Kedisan Kintamani dalam menjaga kebersihan lingkungan sekitar mereka.

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    Journal of Sosial Science
    Article . 2023 . Peer-reviewed
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      Journal of Sosial Science
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    Authors: Elżbieta Drążkiewicz;

    As the coronavirus started to spread in Ireland, the epidemiological data became the most sought-after information in the country This article will examine the ways in which COVID-19 redefined the intimacies of the relationships that health professionals and the members of the public have with medical data It will focus on Irish examples and explore how the context of the pandemic turned numbers from abstract cognitive tools into important and affective tenets of social lives that dictated the moral values and conditions of sociality It will examine the role of enumeration and metrics in mediating new forms of intimacy with state and society [ABSTRACT FROM AUTHOR] Copyright of Anthropology in Action is the property of Berghahn Books and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission However, users may print, download, or email articles for individual use This abstract may be abridged No warranty is given about the accuracy of the copy Users should refer to the original published version of the material for the full abstract (Copyright applies to all Abstracts )

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    Anthropology in Action
    Article . 2020 . Peer-reviewed
    License: CC BY NC ND
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    Anthropology in Action
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      Anthropology in Action
      Article . 2020 . Peer-reviewed
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      Anthropology in Action
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    Authors: Evelyn Hartmann; Beate Kümmerer; Lara Oberkircher; Natalio Garbi; +2 Authors
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    Laryngo-Rhino-Otologie
    Conference object . 2022 . Peer-reviewed
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      Laryngo-Rhino-Otologie
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    Authors: de Albuquerque, Lucia Maria Nunes Freire; Turrini, Ruth Natalia Teresa;

    Abstract Objective: To analyze the effects of flower essence bouquets on the signs and symptoms of stress in nursing students. Method: Randomized clinical trial, triple blind, with two groups, flower essence group and placebo group, carried out with 101 nursing students. The groups used the formula for 60 days at a dosage of 4 drops 4 times a day. The outcome was evaluated using the Baccaro Test and the Perceived Stress Scale applied at the beginning and at the end of the intervention. The outcome analysis was performed using the mixed effects model, with 〈 = 5% and the effect size verified by the Cohen’s d test. Results: There was no significant difference between the groups in stress reduction (p > 0.05). Both groups showed a reduction in scale scores (p 0,05), ambos los grupos tuvieron reducción en los scores de las escalas (p 0,05). Ambos os grupos tiveram redução nos escores das escalas (p < 0,001) com tamanho de efeito grande. Houve influência da pandemia COVID-19 na redução dos escores do Teste de Baccaro. Conclusão: A intervenção com terapia floral não foi mais efetiva que o placebo na redução de sinais e sintomas de estresse. Registro Brasileiro de Ensaios Clínicos: UTNU1111–1257–5715.

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: N. Ahmad Aziz; Victor M. Corman; Antje K.C. Echterhoff; Anja Richter; +6 Authors

    ABSTRACTBackgroundAccurate estimates of SARS-CoV-2 seroprevalence are crucial for the implementation of effective public health measures, but are currently largely lacking in regions with low infection rates. This is further complicated by inadequate test performance of many widely used serological assays. We therefore aimed to assess SARS-CoV-2 seroprevalence in a region with low COVID-19 burden, especially focusing on neutralizing antibodies that presumably constitute a major component of acquired immunity.MethodsWe invited all individuals who were enrolled in the Rhineland Study, an ongoing community-based prospective cohort study in people aged 30 years and above in the city of Bonn, Germany (N=5427). Between April 24th and June 30th, 2020, 4771 (88%) of these individuals participated in the serosurvey. Anti-SARS-CoV-2 IgG levels were measured using an ELISA assay, and all positive or borderline results were subsequently examined through both a recombinant immunofluorescent assay and a plaque reduction neutralisation test (PRNT).FindingsSeroprevalence was 0·97% (95% CI: 0·72–1·30) by ELISA and 0·36% (95% CI: 0·21–0·61) by PRNT, and did not vary with either age or sex. All PRNT+ individuals reported having experienced at least one symptom (odds ratio (OR) of PRNT+ for each additional symptom: 1·12 (95% CI: 1·04–1·21)). Apart from living in a household with a SARS-CoV-2 confirmed or suspected person, a recent history of reduced taste or smell, fever, chills/hot flashes, pain while breathing, pain in arms/legs, as well as muscle pain and weakness were significantly associated with the presence of neutralizing antibodies in those with mild to moderate infection (ORs 3·44 to 9·97, all p<0·018).InterpretationOur findings indicate a relatively low SARS-CoV-2 seroprevalence in Bonn, Germany (until June 30th, 2020), with neutralizing antibodies detectable in only one third of those with a positive immunoassay result, implying that almost the entire population in this region remains susceptible to SARS-CoV-2 infection.FundingThe Rhineland Study is predominantly funded through the German Center for Neurodegenerative Diseases (DZNE) by the Federal Ministry of Education and Research (BMBF) and the Ministry of Culture and Science of the German State of North Rhine-Westphalia. The National Consultant Laboratory for Coronaviruses is funded by the Federal Ministry of Health (BMG). No additional funding was received for this seroprevalence study.

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    Authors: Marek Petráš; Ivana Králová Lesná;

    ABSTRACT Immunological memory is the ability of the adaptive immune system to ensure a persistent protective effect after immunization. However, it can also be a limitation to building a sufficient level of protective antibodies specific to new mutations of the virus. It is imperative to bear this phenomenon (called “original antigenic sin”) in mind and make every effort to overcome its inherent pitfalls when updating current and designing new vaccines.

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    Human Vaccines & Immunotherapeutics
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    Human Vaccines & Immunotherapeutics
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      Human Vaccines & Immunotherapeutics
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    Authors: Francisco J. Zapatero-Belinchón; Erik Dietzel; Olga Dolnik; Katinka Döhner; +14 Authors

    Filoviruses infect a wide range of cell types with the exception of lymphocytes. The intracellular proteins cathepsin B and L, two-pore channel 1 and 2, and bona fide receptor Niemann⁻Pick Disease C1 (NPC1) are essential for the endosomal phase of cell entry. However, earlier steps of filoviral infection remain poorly characterized. Numerous plasma membrane proteins have been implicated in attachment but it is still unclear which ones are sufficient for productive entry. To define a minimal set of host factors required for filoviral glycoprotein-driven cell entry, we screened twelve cell lines and identified the nonlymphocytic cell line SH-SY5Y to be specifically resistant to filovirus infection. Heterokaryons of SH-SY5Y cells fused to susceptible cells were susceptible to filoviruses, indicating that SH-SY5Y cells do not express a restriction factor but lack an enabling factor critical for filovirus entry. However, all tested cell lines expressed functional intracellular factors. Global gene expression profiling of known cell surface entry factors and protein expression levels of analyzed attachment factors did not reveal any correlation between susceptibility and expression of a specific host factor. Using binding assays with recombinant filovirus glycoprotein, we identified cell attachment as the step impaired in filovirus entry in SH-SY5Y cells. Individual overexpression of attachment factors T-cell immunoglobulin and mucin domain 1 (TIM-1), Axl, Mer, or dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) rendered SH-SY5Y cells susceptible to filovirus glycoprotein-driven transduction. Our study reveals that a lack of attachment factors limits filovirus entry and provides direct experimental support for a model of filoviral cell attachment where host factor usage at the cell surface is highly promiscuous. CA extern

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    Viruses
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    Viruses
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      Viruses; Universitätsbibliographie, Universität Duisburg-Essen
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